32 research outputs found

    Maize germ and bran for value addition : high fiber bakery and confectionery products

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    The research aimed to add value to maize germ and bran, to develop improved products for human consumption, and to contribute to food security and human nutrition. Of low economic value, maize germ and bran currently dominate the livestock feed sector. However, with careful handling these processing by-products could be made to impact the confectionery and bakery sector. By incorporating them into bakery and confectionery products, nutritional value would be enhanced. The Ministry of Health and Uganda National Bureau of Standards can develop, implement and enforce standards for a positive impact on Food and Nutrition Policy

    Antimicrobial Activity of Lactic Acid Bacteria Starters against Acid Tolerant, Antibiotic Resistant, and Potentially Virulent E. coli Isolated from a Fermented Sorghum-Millet Beverage

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    Bacterial contamination of fermented foods is a serious global food safety challenge that requires effective control strategies. This study characterized presumptive E. coli isolated from Obushera, a traditional fermented cereal beverage from Uganda. Thereafter, the antimicrobial effect of lactic acid bacteria (LAB) previously isolated from Obushera, against the E. coli, was examined. The presumptive E. coli was incubated in brain heart infusion broth (pH = 3.6) at 25°C for 48 h. The most acid-stable strains were clustered using (GTG)5 rep-PCR fingerprinting and identified using 16S rRNA sequencing. E. coli was screened for Shiga toxins (Stx 1 and Stx 2) and Intimin (eae) virulence genes as well as antibiotic resistance. The spot-on-the-lawn method was used to evaluate antimicrobial activity. Eighteen isolates were acid stable and are identified as E. coli, Shigella, and Lysinibacillus. The Stx 2 gene and antibiotic resistance were detected in some E. coli isolates. The LAB were antagonistic against the E. coli. Lactic acid bacteria from traditional fermented foods can be applied in food processing to inhibit pathogens. Obushera lactic acid bacteria could be used to improve the safety of fermented foods

    Probiotic Potential of Lactic Acid Starter Cultures Isolated from a Traditional Fermented Sorghum-Millet Beverage

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    The purpose of this study was to establish the probiotic potential of lactic acid bacteria (LAB) starter cultures, Lb. plantarum MNC 21, L. lactis MNC 24, and W. confusa MNC 20, isolated from a traditionally fermented sorghum-millet beverage from Uganda. The cultures were examined for tolerance to acid and bile salts, bile salt hydrolase (BSH) activity, antibiotic susceptibility, biogenic amine production, mucin degradation, hydrophobicity, auto-aggregation, adherence to the ileum, coaggregation, and antimicrobial properties against selected pathogenic species. Lb. rhamnosus yoba 2012, a known probiotic, was the reference. The isolates were tolerant to acid (pH = 3) and bile (1%). W. confusa MNC 20 and Lb. plantarum MNC 21 exhibited medium BSH activity (11–15 mm diameter of hydrolysis zone) while L. lactis and Lb. rhamnosus yoba 2012 exhibited low BSH activity (<10 mm diameter of hydrolysis zone). All isolates lacked mucolytic activity. Lb. plantarum MNC 21 and W. confusa MNC 20 produced agmatine. The candidate and reference microorganisms were resistant to 10 of 21 and 5 of 21 antibiotics, respectively. The isolates exhibited hydrophobic, auto-aggregation and coaggregation properties. These three properties were exhibited more (p<0.05) by the reference than the potential probiotics. The ability of the potential probiotics to attach onto the goat ileum (7.3–8.0 log cfu/cm2) was comparable to that of Lb. rhamnosus yoba 2012 (7.6 log cfu/cm2). The four LAB inhibited E. coli, S. aureus, and S. enterica to the same extent (p<0.05). The findings indicated potential probiotic activity of the starter cultures. However, further in vivo examination of these isolates is required to confirm their probiotic capabilities

    PREDICTION OF THE LIKELIHOOD OF HOUSEHOLDS FOOD SECURITY IN THE LAKE VICTORIA REGION OF KENYA

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    This paper considers the modeling and prediction of &nbsp;households food security status using a sample of households in the &nbsp;Lake Victoria region of Kenya. A priori expected food security&nbsp; factors and their measurements are given. A binary logistic regression model&nbsp; derived was fitted to thirteen priori expected factors. Analysis of the marginal effects revealed that effecting the use of the seven significant determinants: farmland size, per capita aggregate production, household size, gender of household head, use of fertilizer, use of pesticide/herbicide and education of household head,&nbsp; increase the likelihood of a household being food secure. Finally, interpretations &nbsp;of&nbsp; &nbsp;predicted conditional probabilities, following improvement of significant determinants, &nbsp;are given

    National food safety control systems in Sub-Saharan Africa: Does Uganda's aquaculture control system meet international requirements

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    Stringent food safety requirements set by developed country markets, which require exporting countries to establish effective national food control systems (NFCS) that guarantee safety of the products to the market, pose a challenge to Sub-Saharan countries in development of aquaculture products as alternative exports following the decline of capture fisheries. In the study, four components of Uganda's NFCS including legislation, competent authority, inspection services, and laboratory services were evaluated for compliance with FAO/WHO, European Union (EU), and the United States (US) market recommendations for guaranteeing aquaculture product safety. Using a checklist, component elements were benchmarked and scored, and components ranked for compliance with the recommendations. On a scale of 0-5, where 0 denotes none, 1 very low, 2 low, 3 some, 4 almost total, and 5 full compliance, only laboratory services had a barely acceptable score of 3.3 (some compliance). The rest including legislation which is central in setting the level of controls by the other three components scored below three, and the combined score for all components was only 2.2, indicating that Uganda's NFCS was still short of the requirements to allow entrepreneurs to access markets in the EU and other developed countries. The low score is partly attributed to the dynamics of this country's fledgling aquaculture industry and the rapidly evolving food safety requirements in the international markets.National food control system Aquaculture Compliance International requirements Uganda Sub-Saharan Africa

    PREDICTION OF THE LIKELIHOOD OF HOUSEHOLDS FOOD SECURITY IN THE LAKE VICTORIA REGION OF KENYA

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    <p class="MsoBodyText" style="margin: 0in 0in 0pt;"><span style="font-size: small; font-family: Times New Roman;">This paper considers the modeling and prediction of <span style="mso-spacerun: yes;">&nbsp;</span>households food security status using a sample of households in the <span style="mso-spacerun: yes;">&nbsp;</span>Lake Victoria region of Kenya. A priori expected food security<span style="mso-spacerun: yes;">&nbsp; </span>factors and their measurements are given. A binary logistic regression model<span style="mso-spacerun: yes;">&nbsp; </span>derived was fitted to thirteen priori expected factors. Analysis of the marginal effects revealed that effecting the use of the seven significant determinants: farmland size, per capita aggregate production, household size, gender of household head, use of fertilizer, use of pesticide/herbicide and education of household head,<span style="mso-spacerun: yes;">&nbsp; </span>increase the likelihood of a household being food secure. Finally, interpretations <span style="mso-spacerun: yes;">&nbsp;</span>of<span style="mso-spacerun: yes;">&nbsp; </span><span style="mso-spacerun: yes;">&nbsp;</span>predicted conditional probabilities, following improvement of significant determinants, <span style="mso-spacerun: yes;">&nbsp;</span>are given. </span></p
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