1 research outputs found
Structurally Ordered Nanowire Formation from Co-Assembly of DNA Origami and Collagen-Mimetic Peptides
We describe the co-assembly of two
different building units: collagen-mimetic
peptides and DNA origami. Two peptides <b>CP</b><sup><b>++</b></sup> and <b>sCP</b><sup><b>++</b></sup> are designed
with a sequence comprising a central block (Pro-Hyp-Gly) and two positively
charged domains (Pro-Arg-Gly) at both N- and C-termini. Co-assembly
of peptides and DNA origami two-layer (<b>TL</b>) nanosheets
affords the formation of one-dimensional nanowires with repeating
periodicity of ∼10 nm. Structural analyses suggest a face-to-face
stacking of DNA nanosheets with peptides aligned perpendicularly to
the sheet surfaces. We demonstrate the potential of selective peptide-DNA
association between face-to-face and edge-to-edge packing by tailoring
the size of DNA nanostructures. This study presents an attractive
strategy to create hybrid biomolecular assemblies from peptide- and
DNA-based building blocks that takes advantage of the intrinsic chemical
and physical properties of the respective components to encode structural
and, potentially, functional complexity within readily accessible
biomimetic materials