25 research outputs found
Plasma contents (µmol/L) of neutral amino acids in Huanjiang mini-piglets with HBW<sup>1</sup> and LBW<sup>2</sup>.
a-d<p>Values within a row without a common superscript letter differ (<i>P</i><0.05).</p>1<p>HBW, high birth weight;</p>2<p>LBW, low birth weight.</p
Liver contents (%) of neutral amino acids in Huanjiang mini-piglets with HBW<sup>1</sup> and LBW <sup>2</sup>.
a-e<p>Values within a row without a common superscript letter differ (<i>P</i><0.05).</p>1<p>HBW, high birth weight;</p>2<p>LBW, low birth weight.</p
Antibodies and dilution used for Western blot analyses.
<p>Antibodies and dilution used for Western blot analyses.</p
Protein abundances of jejunum B<sup>0</sup>AT1 (A) and ASCT2 (B) in sucking Huanjiang mini-piglets with HBW<sup>1</sup> and LBW<sup>2</sup> from days 0 to 21 of age.
<p>Samples were obtained from Huanjiang mini-pigs on Days 0, 7, 14 and 21 of age, respectively. The protein amounts of B0AT1 and ASCT2 were normalized using β-actin as an inner control. Values without a common letter on bars differ (<i>P</i><0.05). The <i>P</i> value showed the effects of age, BW, and age and BW interaction on protein expressiong of B0AT1 and ASCT2. Data are expressed as mean ± SD, n = 5. <sup>1</sup>HBW, high birth weight; <sup>2</sup>LBW, low birth weight.</p
Image_1_Zearalenone Exposure Enhanced the Expression of Tumorigenesis Genes in Donkey Granulosa Cells via the PTEN/PI3K/AKT Signaling Pathway.jpeg
<p>Zearalenone (ZEA) is a natural contaminant existing in food and feed products that exhibits a negative effect on domestic animals’ reproduction. Donkeys possess high economic value in China and are at risk of exposure to ZEA. However, few information is available on ZEA-induced toxicity and no report on toxicity in donkeys can be found in scientific literature. We investigated the biological effects of ZEA exposure on donkey granulosa cells (dGCs) by using RNA-seq analysis. ZEA at 10 and 30 μM were administered to GCs within 72 h of in vitro culture. ZEA at 10 μM significantly altered the tumorigenesis associated genes in dGCs. Exposure to 10 and 30 μM ZEA treatment significantly reduced mRNA expression of PTEN, TGFβ, ATM, and CDK2 genes, particularly, the ZEA treatment significantly increased the expression of PI3K and AKT genes. Furthermore, immunofluorescence, RT-qPCR, and Western blot analysis verified the gene expression of ZEA-exposed GCs. Collectively, these results demonstrated the deleterious effect of ZEA exposure on the induction of ovarian cancer related genes via the PTEN/PI3K/AKT signaling pathway in dGCs in vitro.</p
Image_3_Zearalenone Exposure Enhanced the Expression of Tumorigenesis Genes in Donkey Granulosa Cells via the PTEN/PI3K/AKT Signaling Pathway.jpeg
<p>Zearalenone (ZEA) is a natural contaminant existing in food and feed products that exhibits a negative effect on domestic animals’ reproduction. Donkeys possess high economic value in China and are at risk of exposure to ZEA. However, few information is available on ZEA-induced toxicity and no report on toxicity in donkeys can be found in scientific literature. We investigated the biological effects of ZEA exposure on donkey granulosa cells (dGCs) by using RNA-seq analysis. ZEA at 10 and 30 μM were administered to GCs within 72 h of in vitro culture. ZEA at 10 μM significantly altered the tumorigenesis associated genes in dGCs. Exposure to 10 and 30 μM ZEA treatment significantly reduced mRNA expression of PTEN, TGFβ, ATM, and CDK2 genes, particularly, the ZEA treatment significantly increased the expression of PI3K and AKT genes. Furthermore, immunofluorescence, RT-qPCR, and Western blot analysis verified the gene expression of ZEA-exposed GCs. Collectively, these results demonstrated the deleterious effect of ZEA exposure on the induction of ovarian cancer related genes via the PTEN/PI3K/AKT signaling pathway in dGCs in vitro.</p
Image_2_Zearalenone Exposure Enhanced the Expression of Tumorigenesis Genes in Donkey Granulosa Cells via the PTEN/PI3K/AKT Signaling Pathway.jpeg
<p>Zearalenone (ZEA) is a natural contaminant existing in food and feed products that exhibits a negative effect on domestic animals’ reproduction. Donkeys possess high economic value in China and are at risk of exposure to ZEA. However, few information is available on ZEA-induced toxicity and no report on toxicity in donkeys can be found in scientific literature. We investigated the biological effects of ZEA exposure on donkey granulosa cells (dGCs) by using RNA-seq analysis. ZEA at 10 and 30 μM were administered to GCs within 72 h of in vitro culture. ZEA at 10 μM significantly altered the tumorigenesis associated genes in dGCs. Exposure to 10 and 30 μM ZEA treatment significantly reduced mRNA expression of PTEN, TGFβ, ATM, and CDK2 genes, particularly, the ZEA treatment significantly increased the expression of PI3K and AKT genes. Furthermore, immunofluorescence, RT-qPCR, and Western blot analysis verified the gene expression of ZEA-exposed GCs. Collectively, these results demonstrated the deleterious effect of ZEA exposure on the induction of ovarian cancer related genes via the PTEN/PI3K/AKT signaling pathway in dGCs in vitro.</p
The relative expression of IL-1β, IL-6 and TNF-α mRNA in jejunal mucosa.
<p>Piglets challenged by enterotoxigenic <i>Escherichia</i> coli were fed either a control diet (A group, n = 6), or control diet plus chlortetracycline (B group, n = 6) or control diet plus chitosan (C group, n = 6) for 21 days and 6 pigs per treatment were killed to excise the jejunum.</p
Serum IL-1β, IL-6 and TNF-α levels in weaned piglets.
1<p>A group means the control group, B group means the chlortetracycline group, C group means the COS group.</p