1 research outputs found
Antibodies Among Healthy Population of Developing Countries Against Enterohaemorrhagic Escherichia coli O157:H7
In Thailand, no reports are available on Escherichia coli serotype
O157:H7, a causative agent of severe bloody diarrhoea, sometimes
associated with haemolytic-uraemic syndrome and thrombotic
thrombo-cytopenic purpura. The reason for the non-identification of
infection due to E. coli O157 in this country and in other developing
countries has not been rigorously discussed. The aim of this study was
to determine the humoral response against the infectious organism. The
IgM and IgG antibody responses against E. coli O157 lipopolysaccharide
were studied using indirect enzyme-linked immunosorbent assay. Three
hundred and thirty-two serum samples obtained from healthy blood donors
and patients with diseases unrelated to diarrhoea were investigated.
With a cut-off value of mean +2 SDs for each age-group, the frequency
of the IgM and IgG responses to O157 lipopolysaccharide was 11.74% (39
of 332 samples) and 22.59% (75 of 332 samples) respectively.
Furthermore, agglutination test of 173 subjects revealed titres ranging
from 10 to 40 in all the samples. The results suggest possible exposure
of the Thai population to cross-reacting antigens from other intestinal
organisms in addition to infection due to E. coli O157:H7