Free Boundary Minimal Surfaces in the Unit Three-Ball via Desingularization of the Critical Catenoid and the Equatorial Disk

We construct a new family of high genus examples of free boundary minimal surfaces in the Euclidean unit 3-ball by desingularizing the intersection of a coaxial pair of a critical catenoid and an equatorial disk. The surfaces are constructed by singular perturbation methods and have three boundary components. They are the free boundary analogue of the Costa-Hoffman-Meeks surfaces and the surfaces constructed by Kapouleas by desingularizing coaxial catenoids and planes. It is plausible that the minimal surfaces we constructed here are the same as the ones obtained recently by Ketover using the min-max method.Comment: 45 pages, 10 figure

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Mouse ­Behavior Data for Continuous Authentication

<div>The data consist of mouse dynamics information from 28 subjects who each accomplished at least 30 data sessions. Each session consists of about thirty minutes of a user’s mouse activity data (around 3000 mouse operations).</div

Mouse ­Behavior Data for Static Authentication

<div>The data consist of mouse dynamics information from 56 subjects, each of who accomplish a fixed mouse ­operating pattern 200 times.</div

Genome-wide recombination rate variation in a recombination map of cotton

<div><p>Recombination is crucial for genetic evolution, which not only provides new allele combinations but also influences the biological evolution and efficacy of natural selection. However, recombination variation is not well understood outside of the complex species’ genomes, and it is particularly unclear in <i>Gossypium</i>. Cotton is the most important natural fibre crop and the second largest oil-seed crop. Here, we found that the genetic and physical maps distances did not have a simple linear relationship. Recombination rates were unevenly distributed throughout the cotton genome, which showed marked changes along the chromosome lengths and recombination was completely suppressed in the centromeric regions. Recombination rates significantly varied between A-subgenome (At) (range = 1.60 to 3.26 centimorgan/megabase [cM/Mb]) and D-subgenome (Dt) (range = 2.17 to 4.97 cM/Mb), which explained why the genetic maps of At and Dt are similar but the physical map of Dt is only half that of At. The translocation regions between A02 and A03 and between A04 and A05, and the inversion regions on A10, D10, A07 and D07 indicated relatively high recombination rates in the distal regions of the chromosomes. Recombination rates were positively correlated with the densities of genes, markers and the distance from the centromere, and negatively correlated with transposable elements (TEs). The gene ontology (GO) categories showed that genes in high recombination regions may tend to response to environmental stimuli, and genes in low recombination regions are related to mitosis and meiosis, which suggested that they may provide the primary driving force in adaptive evolution and assure the stability of basic cell cycle in a rapidly changing environment. Global knowledge of recombination rates will facilitate genetics and breeding in cotton.</p></div

Investigation of Cross-Contamination and Misidentification of 278 Widely Used Tumor Cell Lines

<div><p>In recent years, biological research involving human cell lines has been rapidly developing in China. However, some of the cell lines are not authenticated before use. Therefore, misidentified and/or cross-contaminated cell lines are unfortunately commonplace. In this study, we present a comprehensive investigation of cross-contamination and misidentification for a panel of 278 cell lines from 28 institutes in China by using short tandem repeat profiling method. By comparing the DNA profiles with the cell bank databases of ATCC and DSMZ, a total of 46.0% (128/278) cases with cross-contamination/misidentification were uncovered coming from 22 institutes. Notably, 73.2% (52 out of 71) of the cell lines established by the Chinese researchers were misidentified and accounted for 40.6% of total misidentification (52/128). Further, 67.3% (35/52) of the misidentified cell lines established in laboratories of China were HeLa cells or a possible hybrid of HeLa with another kind of cell line. Furthermore, the bile duct cancer cell line HCCC-9810 and degenerative lung cancer Calu-6 exhibited 88.9% match in the ATCC database (9-loci), indicating that they were from the same origin. However, when we used 21-loci to compare these two cell lines with the same algorithm, the percent match was only 48.2%, indicating that these two cell lines were different. The SNP profiles of HCCC-9810 and Calu-6 also revealed that they were different cell lines. 150 cell lines with unique profiles demonstrated a wide range of <i>in vitro</i> phenotypes. This panel of 150 genomically validated cancer cell lines represents a valuable resource for the cancer research community and will advance our understanding of the disease by providing a standard reference for cell lines that can be used for biological as well as preclinical studies.</p></div

The linear relationship between recombination rates and density of <i>NBS-LRR</i> genes.

<p>The linear relationship between recombination rates and density of <i>NBS-LRR</i> genes. Error bars represent ± standard error.</p

The numbers of cross-contamination in the Chinese model.

<p>The numbers of cross-contamination in the Chinese model.</p

24 Loci SNP genotype profile for HCCC-9810 and Calu-6 cell line.

<p>24 Loci SNP genotype profile for HCCC-9810 and Calu-6 cell line.</p