1 research outputs found
Photoelectrochemical Aptasensing of Kanamycin Using Visible Light-Activated Carbon Nitride and Graphene Oxide Nanocomposites
Photoactive material and recognition
element are two crucial factors
which determine the sensitivity and selectivity of the photoelectrochemical
(PEC) sensor. Herein we developed a novel PEC aptamer sensor for the
specific detection of kanamycin using water-dispersible graphite-like
carbon nitride (w-<i>g</i>-C<sub>3</sub>N<sub>4</sub>) as
visible light-active material and aptamer as the biorecognition element.
While a suitable amount of graphene oxide (GO) was doped in w-<i>g</i>-C<sub>3</sub>N<sub>4</sub>, the visible light photocurrent
response was enhanced, which was beneficial to the construction of
PEC sensor. On the other hand, the large specific surface area and
Ï€-conjugated structure of GO/w-<i>g</i>-C<sub>3</sub>N<sub>4</sub> provided an excellent platform for immobilizing the
kanamycin-binding DNA aptamer on the surface of the sensor via π–π
stacking interaction. On such a sensor, the capture of kanamycin molecules
by aptamer resulted in increased photocurrent. The PEC response of
the sensor was found to be linearly proportional to the concentration
of kanamycin in the range from 1 nM to 230 nM with a detection limit
(3S/N) of 0.2 nM. Moreover, the proposed sensor displayed high selectivity,
good reproducibility, and high stability, demonstrating the successful
combination of GO/w-<i>g</i>-C<sub>3</sub>N<sub>4</sub> with
aptamer in fabricating high performance PEC sensors