Sequential FISH and GISH karyotypes of M8003 (a), Austrian rye (b), N9116H (c) and N9116M (d).

<p>(a, c, d) 4',6-diamidino-2-phenylindole (DAPI), blue fluorescence; rye genomic DNA and Oligo-pTa535, red fluorescence; Oligo-pSc119.2, green fluorescence. (b) Oligo-pSc119.2, red fluorescence. Alterations of wheat chromosomes were indicated in white box.</p

Morphologic traits of hexaploid triticales N9116H/M and their parents.

<p>Morphologic traits of hexaploid triticales N9116H/M and their parents.</p

Breeding scheme showing the development of N9116H and N9116M.

<p>Breeding scheme showing the development of N9116H and N9116M.</p

Morphologic traits of M8003, Austrian rye, N9116H and N9116M.

<p>a Plant of M8003, Austrian rye, N9116M and N9116H; b Spikes of M8003, Austrian rye, N9116M and N9116H; c Spikelets and kernels of M8003, Austrian rye, N9116H and N9116M; d Resistance of M8003, Austrian rye, N9116H and N9116M for powdery mildew and stripe rust. 1–4 in figures represent M8003, Austrian rye, N9116M and N9116H, respectively.</p

Storage protein variations in hexaploid titicales N9116H and N9116M.

<p>1–6 represent Xiaoyan No.6, Chinese Spring, M8003, Austrian rye, N9116H and N9116M, respectively. Black arrow, position of inherited HMW-GS subunit of Austrian rye; red arrow, novel HMW-GS subunit emerged in N9116H and N9116M; red triangle, novel secalins emerged in N9116H; red box, HMW-GS variations in N9116H and N9116M.</p

Fluorescence <i>in situ</i> hybridization (FISH) analysis using Oligo-pTa535 (red), Oligo-pSc119.2 as probes on root tip metaphase chromosomes of M11003-3-1-15-8 (a), Genomic <i>in situ</i> hybridization (GISH) analysis using <i>L</i>. <i>mollis</i> genomic DNA (green) as probe on root tip metaphase chromosomes of M11003-3-1-15-8.

<p>Chromosomes were counterstained with DAPI (blue). The yellow arrows indicate the introduced <i>L</i>. <i>mollis</i> chromosomes in M11003-3-1-15-8.</p

Genomic <i>in situ</i> hybridization (GISH) analysis using <i>L</i>. <i>mollis</i> genomic DNA (green) as probe on root tip metaphase chromosomes of M11003-3-1-15-8.

<p>Chromosomes were counterstained with PI (red).</p

Nullisomic-tetrasomic analysis of M11003-3-1-15-8.

<p>The arrow indicates an <i>L</i>. <i>mollis</i> specific band. M DL2000, 1 7182, 2 <i>L</i>.<i>mollis</i>, 3 M11003-3-1-15-8, 4 M47, 5 CS, 6 CSN7AT7D, 7 CSN7BT7A, 8 CSN7DT7B; a TNAC1826-<i>Taq</i>I, b TNAC1957-<i>Taq</i>I, c TNAC1926-<i>Hae</i>III, d TNAC1926-<i>Taq</i>I.</p

Fluorescence <i>in situ</i> hybridization (FISH) analysis using Oligo-pTa535 (red), Oligo-pSc119.2 (green) as probes on root tip metaphase chromosomes of 7182.

<p>Chromosomes were counterstained with DAPI (blue). The pair of wheat chromosomes 7<b>D</b> was indicated in a small white rectangular box.</p

EST-STS markers results of M11003-3-1-15-8.

<p>The arrow indicates an <i>L</i>. <i>mollis</i> specific band. M DL2000, 1 7182, 2 <i>L</i>.<i>mollis</i>, 3 M11003-3-1-15-8. <i>BE482781-</i>7<b>A</b>L 7<b>B</b>L 7<b>D</b>L, <i>BE404955-</i>7<b>A</b> 7<b>B</b>S 7<b>D</b>.</p