Additional file 9: of Transcriptome profile analysis of cell proliferation molecular processes during multicellular trichome formation induced by tomato Wo v gene in tobacco

Expression patterns of the homologues of the known trichome-related genes in transgenic tobacco plants. (XLS 28 kb

Relative transcript levels of the oxidative stress-related genes in the tomato lesioned leaves.

<p>The expression levels of oxidative related genes <i>CAT</i>, <i>SOD</i>, <i>cAPX</i>, and <i>chlAPXs</i> in the tomato lesioned leaves were measured by real-time RT-PCR. Data were normalized against <i>Actin</i>.</p

Expression analysis of four <i>SlGMPs</i> and other AsA biosynthesis-related genes in <i>SlGMP3</i> transgenic plants.

<p>(A) RT-PCR analysis of <i>SlGMP3</i> expression in the young leaves of two <i>SlGMP3</i>-OX lines (upper panel) and 17 <i>SlGMP3</i> RNAi lines (bottom panel). The PCR circle numbers are indicated on the right. (B) Relative expression analysis of four members of <i>SlGMP</i> gene family in the young leaves of lines OX6 and OX19 (left) and lines KD7 and KD17 (right) via real-time RT-PCR. (C) Relative expression analysis of AsA biosynthesis-related genes in the young leaves of lines OX6 and KD17 via real-time RT-PCR. Data were obtained by normalizing against <i>Actin</i> and shown as a percentage of wild-type plants.</p

Photosynthetic system was impaired in leaves of <i>SlGMP2/3</i>-KD tomato plants.

<p>(A) Relative transcript levels of the photosynthesis-related genes in wild-type leaves and KD17 necrotic leaves were assayed via real-time RT-PCR. Data were normalized against <i>Actin</i> and shown as a percentage of wild-type plants. (B) Net photosynthesis rates of KD7 and KD17 necrotic leaves and wild-type leaves were measured using a portable photosynthetic system (CIRAS-2, PP System, USA). Data are presented as mean ± SD (N = 4) from triplicate independent measurements. Asterisk indicates significant differences from the control (P>0.95).</p

Improvement of the photo-oxidative stress tolerance in tomato plants via over-expressing <i>SlGMP3</i>.

<p>Chlorophyll content (A) and MDA content (B) in the fourth leaf of the MV-treated and untreated plants measured at 7 d post-treatment. Data are presented as mean ± SD (N = 6) from triplicate independent measurements. Asterisk indicates significant differences from the control (<i>P</i>>0.95).</p

Relative expression analysis of four members of <i>SlGMP</i> gene family in various tissues of tomato variety Ailsa Craig.

<p>R: root, S: stem, L: leaf, Fl: flower, GF: green fruit, BF: breaker fruit, and RF: red fruit. Data obtained by real-time RT-PCR were normalized against <i>Actin</i>.</p

AsA content analysis in <i>SlGMP3</i> transgenic plants.

<p>(A) Total AsA contents in the mature leaves (left) and red fruits (right) of lines OX6 and OX19. (B) Total AsA contents in the mature leaves (left) and immature green fruits (IMF) and breaker fruits (BF) (right) of lines KD7 and KD17. As the experiments of (A) and (B) were carried at two separate times, there were some differences in AsA contents in leaves of the wild-type tomato plants. Data are presented as mean ± SD of four independent plants per line. Asterisk indicates significant differences from the control (<i>P</i>>0.95).</p

Relative transcript levels of the pathogenesis-related genes in the tomato lesioned and normal leaves and fruits.

<p>Expression analysis of the pathogenesis-related genes <i>PR1b1</i>, <i>PR-P2</i>, and <i>PR-P6</i> was performed in necrotic mature leaves (A), non-necrotic normal young leaves (B) and immature green fruits (C) of lines KD7 and KD17 by real-time RT-PCR. Data were normalized against <i>Actin</i>.</p

Phenotype comparisons of <i>SlGMP2/3</i>-KD and wild-type plants.

<p>(A) Altered cotyledon morphology of <i>SlGMP2/3</i>-KD vs wild-type plants. KD7 and KD17 cotyledons developed lesions (left) at three weeks post germination and accelerated senescence (right) compared with the wild type. (B) Seedlings of wild-type (left) and KD7 (right) plants. The bottom leaves of KD7 plant started to wilt. (C) Plant morphology of the three-month-old wild-type (left) and KD7 (right) plants. Middle and bottom leaves of KD7 plants became dry wilted. (D) Lesion formation on KD7 and KD17 leaves. The leaf lesion areas on the two-month-old plants of KD7 and KD17 (upper panel) are consistent with the areas of dead cells revealed through trypan blue staining (bottom panel). (E) H₂O₂ accumulation in the leaves of KD7 and KD17. H₂O₂ accumulation was revealed via DAB staining in leaves without (upper panel) and with (lower panel) visible lesions from the two-month-old plants of KD7 and KD17. (F) Tomato fruits harvested from four-month-old wild-type and transgenic plants.</p