Adverse events in 5-year follow-up.

<p>Adverse events in 5-year follow-up.</p

Risk factors for death-censored graft loss (5 years).

<p>HR, hazard ratio; CI, confidence interval.</p><p>Risk factors for death-censored graft loss (5 years).</p

Risk factors for patient death (5 years).

<p>Risk factors for patient death (5 years).</p

Causes of graft loss and mortality (5 years).

<p>ARF, acute renal failure; PNF, primary no function.</p><p>Causes of graft loss and mortality (5 years).</p

Recipient baseline characteristics (N = 166).

<p>Recipient baseline characteristics (N = 166).</p

Patient survival, overall and death-censored graft survival.

<p>Patient survival, overall and death-censored graft survival.</p

The usage frequency of each functional V/J gene for TCRB and IGH repertoires.

<p>(a-b) The usage frequency of each functional V gene in TCRB and IGH repertoires, respectively. (c-d) The usage frequency of each functional J gene in TCRB and IGH repertoire, respectively.</p

A comprehensive profiling of T- and B-lymphocyte receptor repertoires from a Chinese-origin rhesus macaque by high-throughput sequencing

<div><p>Due to the close genetic background, high similarity of physiology, and susceptibility to infectious and metabolic diseases with humans, rhesus macaques have been widely used as an important animal model in biomedical research, especially in the study of vaccine development and human immune-related diseases. In recent years, high-throughput sequencing based immune repertoire sequencing (IR-SEQ) has become a powerful tool to study the dynamic adaptive immune responses. Several previous studies had analyzed the responses of B cells to HIV-1 trimer vaccine or T cell repertoire of rhesus macaques using this technique, however, there are little studies that had performed a comprehensive analysis of immune repertoire of rhesus macaques, including T and B lymphocytes. Here, we did a comprehensive analysis of the T and B cells receptor repertoires of a Chinese rhesus macaque based on the 5’—RACE and IR-SEQ. The detailed analysis includes the distribution of CDR3 length, the composition of amino acids and nucleotides of CDR3, V, J and V-J combination usage, the insertion and deletion length distribution and somatic hypermutation rates of the framework region 3 (FR3). In addition, we found that several positions of FR3 region have high mutation frequencies, which may indicate the existence of new genes/alleles that have not been discovered and/or collected into IMGT reference database. We believe that a comprehensive profiling of immune repertoire of rhesus macaque will facilitate the human immune-related diseases studies.</p></div

The usage frequencies of all possible distinct functional V-J pairing in TCRB and IGH repertoires.

<p>(a-b) The frequencies of all possible distinct functional V-J pairing in TCRB and IGH repertoires, respectively. The x axis represents all functional J genes and the y axis represents all functional V genes. The area of the circle is proportional to the frequency of a V-J pairing.</p

Several characteristics of CDR3 in TCRB and IGH repertoires.

<p>(a) The length distribution of CDR3 (at amino acid level, our CDR3 includes the first C and the last F/W). (b-c) The CDR3s were ranked from highest to lowest by their frequency in TCRB and IGH repertoires, respectively. (d) The rarefaction curve of CDR3 in TCRB repertoire (left) and IGH repertoire (right). (e) The composition of twenty kinds of amino acids of CDR3 in TCRA and TCRB repertoires. (f) The composition of twenty kinds of amino acids of CDR3 in IGK, IGL, and IGH repertoires.</p