Multiplex real-time PCR using temperature sensitive primer-supplying hydrogel particles and its application for malaria species identification - Fig 2

<p>(a) Effective capture of primer on supplimer of sPIN particle. In order to see the capturing efficiency, sPIN particles were prepared with matched (red) or mismatched (black) supplimer to primer. After incubation with FAM-modified primer, both of them showed high intensity depending on the injected FAM-primer concentration (filled red and black circle). After rinsing, mismatched sPIN particle lost the fluorescence (empty black circle), meanwhile matched sPIN particle showed consistently remained fluorescence (empty red circle). It was caused by the stable binding between the primer and corresponding supplimer. (b) Positive qPCR graph of each sPIN particle using supplimers with different T_m. (c) Negative qPCR graph of each sPIN particle with supplimers of different T_m. (d) Fluorescent signal subtracted No Template Control (NTC) signal from Positive Template Control (PTC) showing regular S-shaped curves.</p

PCR reaction with sPIN particle and multiplex qPCR.

<p>PCR reaction with sPIN particle and multiplex qPCR.</p