The Effect of Banana Rhizosphere Chemotaxis and Chemoattractants on Bacillus velezensis LG14-3 Root Colonization and Suppression of Banana Fusarium Wilt Disease

Fusarium oxysporum f. sp. cubense (Foc) causes banana Fusarium wilt disease, which is a destructive soil-borne disease. Many plants can recruit rhizosphere microorganisms using their root exudates, thereby shaping the rhizosphere microbiome to resist pathogen infection. Therefore, this study was conducted to explore the role of root exudates in the process of biocontrol strain colonization and resistance to pathogens. In this study, the banana root exudates used as chemoattractants were obtained by hydroponics. Bacillus velezensis strain LG14-3 was isolated from the infected area of the root system of banana and showed significant chemotaxis to banana root exudates and strong inhibition of Fusarium oxysporum f. sp. cubense. Further analysis found that LG14-3 showed chemotaxis toward the components of banana root exudates, such as citric acid, succinic acid, glycine, D-galactose and D-maltose, and glycine and citric acid, which resulted in more significant chemotaxis of LG14-3. Moreover, banana root exudates enhanced the swarming motility and biofilm formation of LG14-3. Pot experiments showed that glycine and citric acid enhanced the colonization ability of Bacillus velezensis LG14-3 in the banana rhizosphere and reduced the disease severity index of banana fusarium wilt. Glycine and citric acid enhanced the growth-promoting ability of LG14-3 under pathogen stress. Our results showed that the addition of chemotactic substances enhanced the biocontrol potential of Bacillus velezensis LG14-3 to prevent banana Fusarium wilt

Table_1_Association between oxidative balance score and sarcopenia in US adults: NHANES 2011–2018.XLSX

BackgroundSarcopenia, a condition characterized by diminished skeletal muscle mass, strength, and function, accompanied by inflammation and oxidative stress, remains an area of limited exploration concerning its correlation with the Oxidative Balance Score (OBS).MethodsLeveraging data from the 2011–2018 National Health and Nutrition Examination Survey (NHANES), we meticulously examined 16 dietary and four lifestyle factors to derive the OBS. Adjusting appendicular skeletal muscle mass (ASM) by body mass index (BMI) served as the designated marker for sarcopenia. To scrutinize the association between OBS and sarcopenia, we conducted weighted logistic regression and engaged in sensitivity analysis. Furthermore, we implemented subgroup analysis and interaction tests to gain comprehensive insights into the relationship across diverse populations.ResultsIn a sample comprising 6,677 individuals aged 20–59, logistic regression illuminated a negative association between OBS and sarcopenia [OR = 0.942 (0.920, 0.964), p ConclusionOur study posits a perceptible link between OBS and the prevalence of sarcopenia among American adults.</p

A meta-analysis: Does vitamin D play a promising role in sleep disorders?

BACKGROUND Sleep disorders, one of the most common problems in the general population, have been related to a series of harmful health consequences. Vitamin D appears to be associated with sleep disorders. However, the difference in vitamin D levels between sleep disorder subjects and people without a sleep disorder is unclear. Simultaneously, the influence of vitamin D replenishment on sleep disorders remains controversial. METHODS PubMed, MEDLINE, Web of Science, and Cochrane Library were searched for literatures published until October 2019. Using a random effects model, a meta-analysis was conducted to calculate the standard mean difference to evaluate the difference in vitamin D concentrations between sleep disorder subjects and normal people and the efficacy of vitamin D supplementation on sleep disorders. RESULTS Our study found that the serum vitamin D levels in the sleep disorder subjects were lower than that in the normal people (SMD = −0.75 ng/ml, 95% CI = −0.93, −0.57 ng/ml). Moreover, the Pittsburgh Sleep Quality Index (PSQI)in the subjects with vitamin D supplementation was lower than that in the controls (SMD = −0.45, 95% CI = −0.76, −0.13). CONCLUSIONS Vitamin D could play a promising role in sleep disorders. More data are required to confirm the efficacy of vitamin D supplementation for improving sleep disorders