1 research outputs found
Identification of a Small Molecule That Turns ON the Pluripotency Gene Circuitry in Human Fibroblasts
A nontransgenic
approach to reprogram mouse somatic cells into
induced pluripotent stem cells using only small molecules got achieved
to propose a potential clinical-friendly cellular reprogramming strategy.
Consequently, the screening and identification of small molecules
capable of inducing pluripotency genes in human cells are increasingly
a focus of research. Because cellular reprogramming is multifactorial
in nature, there is a need for versatile small molecules capable of
modulating the complicated gene networks associated with pluripotency.
We have developed a targeting small molecule called SAHA-PIP comprising
the histone deacetylase inhibitor SAHA and the sequence-specific DNA
binding pyrrole-imidazole polyamides for modulating distinct gene
networks. Here, we report the identification of a SAHA-PIP termed <b>I</b>Ì€ that could trigger genome-wide epigenetic reprogramming
and turn ON the typically conserved core pluripotency gene network.
Through independent lines of evidence, we report for the first time
a synthetic small molecule inducer that target and activate the <i>OCT-3/4</i> regulated pluripotency genes in human dermal fibroblasts