13 research outputs found
Identification of the genus<em> Salmonella</em> and <em>S. typhi</em> by multiplex PCR
[no abstract available
Molecular characterization of drug sensitive and drug resistant strains of Salmonella typhi
[no abstract available
Evaluation of PCR detection of <em>S. typhi</em> DNA in the diagnosis of clinically suspected Typhoid fever
[no abstract available
Role of Molecular Typing in an Outbreak of Salmonella paratyphi A
During the post monsoon season of 1996 an outbreak of human Salmonellosis caused by Salmonella serovar-paratyphi A occurred in New Delhi and had continued for over 2 months. A total of 36 clinically diagnosed enteric-fever cases were reported during this outbreak. The isolates were compared following their characterisation by biotyping, antibiogram-analysis, plasmid-profiling and IS200 probing, to study the relatedness in order to delineate a common source. The study included representative strains from both outbreak (15) and sporadic (7) cases for comparative analysis. Biotyping, antibiogram, whole cell protein-analysis and plasmid-profiling could not discriminate sporadic cases from outbreak strains, suggesting that a single clone/type (PT-1) may be prevalent in our region. In contrast, molecular-typing using IS200-probing revealed 2 clonally related strains circulating during the outbreak, as compared to the unrelated sporadic strains which exhibited considerable genetic diversity. Molecular analysis by IS200-probing, helped to assign an index case which provided a history of later outbreaks, since paratyphi A was repeatedly cultured in later outbreaks also. The study also suggests that genetic rearrangements can occur during the emergence of outbreaks. It reaffirmed the usefulness of IS200-probing in epidemiological investigations of Salmonella enterica serovars
Application of nested Polymerase Chain Reaction for <em>Salmonella enterica</em> v<em>ar. typhi</em> in the diagnosis of Typhoid fever
[no abstract available
<em>Salmonella enterica serovar senftenberg</em> infection in the neonatal intensive care unit
[no abstract available