Phase contrast photomicrographs of breast cancer cell lines at sub confluent and confluent stages.

<p>(A) ARM-H (B) ARM-G (C) ARM-E (D) ARM-X cell lines (magnification x200).</p

IHC stain of ERα in tumor isolated from nude mice.

<p>(A) positive control human breast cancer tissue, (B) tumor from breast cancer cell line ARM-H derived from nude mice, arrows indicate negative mouse nodules.</p

Expression of CD44/CD24 receptors on breast cancer cells.

<p>The plots depict CD44, CD24 and an isotype control antibody staining of ARM-H, ARM-E and control MDA-MB-231. This is one representative experiment of three independent experiments.</p

Representative metaphase (left) and interface (right) from four-breast cancer cell lines.

<p>Each cell nucleus contains <i>HER2</i> signals (red) and centromere 17 signals (green). (A) ARM-H (B) ARM-G (C) ARM-E and (D) SK-BR-3; (E) composite karyotype of chromosome 17 and derivatives in four cell lines; (F) summary table of FISH analyses; (G) number of chromosomes in breast cancer cell lines.</p

Summary of the results for tumorigenicity and percentage of expression of different antigens in breast cancer cell lines.

*<p>Indicates scattered cytoplasmic staining.</p

Breast cancer cell lines express different levels of estrogen receptor and respond differently to estrogen and 4-OHT treatments.

<p>(A) bottom. Western blotting analyses of breast cancer cells for ER expression and top RT-qPCR for cMYC gene expression in RNA extracted from indicated cells treated with 10 nM of E2 for 24 h. (B) Cytotoxicity assay of MCF-7, (C) ARM-E and (D) ARM-H breast cancer cells treated for 3 days with different concentrations of 4-OHT. Left axis represents the relative percentage of live cells treated with 4-OHT compared to medium-treated cells. Similar results were obtained in three independent experiments. Data represents mean ± SD. Student’s t test was used to compare means of treated versus untreated samples with *p<0.05 considered statistically significant.</p

Photomicrographs of H&E stains of human breast cancer cell lines and metastatic tumors from mice.

<p>(A) ARM-H (middle, 50×) shows focal residual skeletal muscle (arrow) almost completely replaced and infiltrated by invasive adenocarcinoma (between asterisks); (right, 200×) the same area shows sheets of markedly atypical and pleomorphic carcinoma cells with numerous mitotic figures in a haphazard growth pattern. (B) ARM-G (middle, 50×) lymph node with deposits of metastatic tumor cells within the peripheral sinuses and parenchyma (arrows); (right, 200×) same lymph node with numerous scattered metastatic tumor cells; tumor cells show enlarged nuclei, significant atypia and pleomorphism when compared to the surrounding normal lymphocytes, arrows indicate deposits of tumor cells. (C) ARM-C (middle, 50×) shows sheets of adenocarcnoma cells (between asterisks) invading into adjacent skeletal muscle (arrow); (right, 200×) view of the same area shows markedly pleomorphic and atypical tumor cells with areas of tumor cell necrosis (arrow). (D) ARM-X (middle, 50×) skin (arrow on epidermis), underlying adnexal structures and dermis completely replaced by invasive adenocarcinoma entrapping sweat glands and normal follicles; (right, 200×) the same section showing the markedly atypical tumor cells with mitoses, pleomorphic nuclei, and high nuclear to cytoplasmic ratio invading the dermis and surrounding adnexal structures (triangle); arrow indicates skin adnexal structures.</p