The RhoA GTPase-activating protein DLC2 modulates RhoA activity and hyperalgesia to noxious thermal and inflammatory stimuli

Deleted in liver cancer 2 (DLC2) is a novel Rho GTPase-activating protein that regulates RhoA activity. DLC2 is ubiquitously expressed in most tissues, including the brain, spinal cord and peripheral nerves, and is thought to be involved in actin cytoskeletal reorganization. Unlike DLC1-deficient mice, DLC2-deficient mice (DLC2 -/-) are viable and without gross anatomical abnormalities. Interestingly, DLC2 -/- mice exhibit hyperalgesia to noxious thermal stimuli and inflammation-inducing chemicals, such as formalin and acetic acid. There was no difference in the structure or morphology of cutaneous or sural nerves between DLC2 +/+ and DLC2 -/- mice. However, sensory nerve conduction velocity in DLC2 -/- mice was significantly higher than that in DLC2 +/+ mice, whereas motor nerve conduction velocity was not affected. After formalin injection, DLC2 -/- mice showed increased RhoA activity in the spinal cord and an increased number of phosphorylated ERK1/2-positive cells. The inflammatory hyperalgesia in DLC2 -/- mice appeared to be mediated through the activation of RhoA and ERK1/2. Taken together, DLC2 plays a key role in pain modulation during inflammation by suppressing the activation of RhoA and ERK to prevent an exaggerated pain response, and DLC2 -/- mice provide a valuable tool for further understanding the regulation of inflammatory pain. Copyright © 2011 S. Karger AG, Basel.postprin

PITX2 transcription factor is overexpressed and involved in the tumorigenicity of ovarian cancer

Free Paper Session - Biomedicine: abstract no. A2

Anti-inflammatory effects of lutein in retinal ischemic injury: in vivo and in vitro studies

postprin

Prevalence of occult hepatitis B infection in a highly endemic area for chronic hepatitis B: A study of a large blood donor population

Background and aims: The aim of the present study was to determine the population prevalence of occult hepatitis B (OHB) infection and its clinical profile in a highly endemic area of chronic hepatitis B virus disease. Methods: OHB was first identified by individual sample testing for hepatitis B surface antigen (HBsAg) followed by nucleic acid testing (NAT) and vice versa for 3044 (cohort 1, stored sera from donation within 1 year) and 9990 (cohort 2, prospective study) blood donors, respectively. OHB was confirmed meticulously by ≥2 out of 3 tests with detectable hepatitis B virus (HBV) DNA using a sensitive standardised assay. Detailed serology and viral load in the serum and liver were studied. Results: The prevalence of OHB was 0.13% (4/3044) and 0.11% (11/9967) for cohort 1 and 2, respectively. In cohort 2, 10 out of 11 OHB samples were positive for anti-HBc (hepatitis B core antigen) antibody (all were immunoglobulin G). Seven had detectable anti-HBs. The serum HBV DNA levels were extremely low (highest 14.1 IU/ml). Of the six donors who underwent liver biopsies, all had normal liver biochemistry, extremely low liver HBV DNA (highest 6.21 copies/cell) and nearly normal liver histology. For those with viral sequence generation, none had the common HBsAg mutant G145R. Conclusions: The prevalence of OHB in a highly endemic area of chronic HBV was very low, thus implying a low impact on transfusion services. To implement universal screening, the high cost of NAT should be taken into account. OHB blood donors had very low HBV replication, and normal liver biochemistry and histology, conferring a favourable prognosis.published_or_final_versio

Path models of quality of life among people with schizophrenia living in the community in Hong Kong

The utilization of quality of life (QOL) in psychiatric community rehabilitation could be enhanced by developing an appropriate conceptual model of QOL. The focus of this study was to construct and test, using path analytic techniques, a pertinent conceptual model of QOL among people with schizophrenia living in the community. A total of 201 participants with schizophrenia living in the community were assessed with regard to their clinical characteristics and QOL. Findings largely supported the proposed model in which community/social functioning was the strongest predictor of QOL, followed by symptom levels. Important implications for the design and implementation of appropriate services in functioning augmentation that resulted in QOL enhancement were highlighted. Further recommendations on both clinical and environmental interventions to promote QOL were suggested. © 2007 Springer Science+Business Media, LLC.link_to_subscribed_fulltex

Clinical characteristics and objective living conditions in relation to quality of life among community-based individuals of schizophrenia in Hong Kong

Quality of life (QOL) has gained importance as an outcome measure for people with schizophrenia living in the community following deinstitutionalization. This study aims at exploring the effects of clinical characteristics and objective living conditions on QOL. In this study, 201 community-based individuals with schizophrenia were recruited from five different types of objective living conditions comprising long stay care home, halfway house, supported hostel/housing, living with family, and living alone. Clinical characteristics including cognitive abilities, symptom levels, and community/social functioning were assessed by the Allen Cognitive Level Screen, the Scales for the Assessment of Negative Symptoms and Positive Symptoms, and the Chinese version of the Multnomah Community Ability Scale respectively. The outcome measure of QOL was measured by the Chinese version of the WHO Quality of Life Measure. Analysis of covariance showed significant differences in community/social functioning, cognitive abilities, and negative symptoms; but not in QOL under different objective living conditions. Further simultaneous multiple regressions found out that community/social functioning was the robust significant predictor of QOL. Yet caution should be noted in making the conclusion with the objective living condition of long stay care home, as it provides a protective element for the perseverance of QOL. © 2006 Springer Science+Business Media B.V.link_to_subscribed_fulltex

Upregulation of PITX2 transcription factor is associated with ovarian tumorigenesis

Poster Presentation - Theme 6: Cancer: 6.08The 15th Research Postgraduate Symposium (RPS 2010), the University of Hong Kong, Hong Kong, China, 1-2 December 2010

PITX2 transcription factor is overexpressed and involved in the tumorigenicity of ovarian cancer

The 22nd Lorne Cancer Conference, Lorne, Victoria, Australia, 11-13 February 2010

Communicating reasoning in Q&A sessions of academic presentations: experts and novices in action

Conference Theme: Transcending Boundaries in Language Learning: Language Arts and ELT Across the CurriculumCommunicating reasoning is an essential part of academic interaction. Yet it is not a commonly addressed aspect of students’ competence development for Q&A sessions, where the attention often focuses on more global skills. There is also a tendency to draw on stocks of interactional wisdom imparted through print and online resources. Despite growing interests in analysing actual speech events such as graduate seminars and press conferences, there is little reported research on academic Q&A sessions. This paper reports on part of a larger study that aims to contribute to meeting the need for the teaching of Q&A skills to be research-informed. The larger study examines the communication behaviours of professors/researchers and university students in videoed Q&A sessions which make up the data. This paper looks specifically into how the two groups of speakers communicate their reasoning in responding to questions. The action of reasoning is analysed adopting mainly conversation analysis methodology, with emphasis placed on identifying interactional patterns rather than analysing the content of the responses. The patterns found in the two groups are compared and pedagogical implications of the findings are explored. The paper also discusses the potential contribution of the findings towards verifying the received wisdoms as well as adding new dimensions to the current understanding of Q&A skills

Characterization of a Karenia papilionacea-like dinoflagellate from the South China Sea

The morphological characteristics of a new isolate of a dorso-ventrally flattened athecate dinoflagellate obtained from Hong Kong (HK) waters is described. Partial sequences of the large subunit ribosomal RNA gene were obtained and a high degree of similarity was observed with various species within the genus Karenia. Phylogenetic comparison suggested the Karenia sp. HK forms a clade with the newly described species K. papffionacea from New Zealand but not with authentic K brevis from North America. Karenia sp. HK is the first K. brevis-like dinoflagellate characterized by molecular cladistics from the South China Sea and the results call for re-examination of the status of other K brevis-like morphotypes described from the region