Trans-Esophageal Echocardiogram (TEE)

<p>TEE view of significant mitral stenosis (thickening of the mitral valve leaflets with doming of the anterior leaflet) with severe spontaneous echo contrast (white arrow).</p

Angiography of the Left Posterior Circulation of the Brain

<p>Angiography (sagittal view) demonstrates complete occlusion of the left vertebral artery (black arrow).</p

Angiography of the Left Anterior Circulation of the Brain

<p>Angiography shows the left internal carotid artery with an occlusion of one of its main branches, the left MCA (arrow shows origin occluded by a thrombus).</p

Diffusion-Weighted Imaging (DWI) of the Brain

<p>Diffusion defect indicating irreversible left hemispheric ischemic damage in the area controlling right hand and leg function.</p

Magnetic Resonance Angiography (MRA)

<p>MRA shows the left MCA occluded by a thrombus at its origin (white arrow). This technique uses MR to demonstrate blood vessels within the central nervous system.</p

Angiography of the Left Anterior Circulation of the Brain—Following Thrombolysis

<p>Antero-posterior view demonstrates a renewed flow in the left internal carotid artery and the left hemisphere.</p

Perfusion-Weighted Imaging (PWI) of the Brain

<p>Perfusion amplified signal mismatch (compared to <a href="http://www.plosmedicine.org/article/info:doi/10.1371/journal.pmed.0030233#pmed-0030233-g001" target="_blank">Figure 1</a>), indicating an ischemic area with abnormal function, but no cellular death, salvageable by reperfusion. </p

<i>In vivo</i> response to heat-treated <i>F. nucleatum</i> bacteria.

<p>(A) FACS analysis of chamber exudates, 2 hours and 24 hours following challenge with heat-killed <i>F. nucleatum</i>. Staining was performed with Phycoerythrin conjugated anti-DX5 mAb. The percentages of GFP⁺ NK cells are indicated. One representative experiment out of three is shown. (B) TNF-α level in chamber exudates of Ncr1<i>^+/+</i> (WT) and Ncr1<i>^gfp/gfp</i> (KO) was determined by ELISA, 2 and 24 hours following challenge with viable and heat-killed <i>F. nucleatum</i>. Data represent absorbance at 650 nm ± SD and are average of two different experiments. * <i>p</i><0.05, ** <i>p</i><0.01.</p

Cytokine profiles in challenged chambers.

<p>TNF-α levels were determined by ELISA in chamber exudates of Ncr1<i>^+/+</i> (WT) and Ncr1<i>^gfp/gfp</i> (KO) mice at baseline (0), 2 h and 24 h following bacterial challenge. Data represent absorbance at 650 nm ± SD and are average of three different experiments. ** <i>p</i><0.01.</p

The infiltration of immune cells into the <i>F. nucleatum</i> challenged chambers is NCR1-independent.

<p>The presence of immune cells; (A) lymphocytes and (B) DC (identified by CD11c) and macrophages (identified by F4/80) in the chambers were analyzed by FACS, 2 hours following the injection of <i>F. nucleatum</i>. The mAb used for staining is indicated in the Y axis. IC is isotype control. The percentage of the various cells and the cell numbers are indicated in each quadrant. One representative experiment out of four is shown.</p