Misregulated genes in the <i>nf-yc triple</i> mutant have ABRE-like promoter elements.

<p>A) ABRE-like motif discovered through MEME analysis. B) Positional distribution of MEME motif within the promoter set. TSS - transcriptional start site. To help assess the relationships between Arabidopsis NF-Y proteins discussed here and below, note that phylogenetic trees were previously published <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0059481#pone.0059481-Siefers1" target="_blank">[9]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0059481#pone.0059481-Gusmaroli1" target="_blank">[76]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0059481#pone.0059481-Gusmaroli2" target="_blank">[77]</a>.</p

Full length NF-YB2 is required for the ABF3 interaction.

<p>Y2H assays were performed between AD:ABF3 and DBD fused to: A) Full length NF-YB2 (AA 1–190), B2HFM (AA 26–121), B2N (AA 1–25), B2C (AA 122–190), B2N+B2HFM (AA 1–122), and B2HFM+B2C (AA 122–190); B) Chimeric constructs - full length NF-YB10 (AA1–228), NF-YB2/NF-YB10, NF-YB10/NF-YB2, NF-YB10N (AA1–27) and NF-YB10C (AA 123–228).</p

ABA related genes are misregulated in <i>NF-Y</i> mutant lines.

<p>Gene expression in 24 hr post light incubation seeds analyzed by quantitative RT-PCR for A) <i>ABI3</i>, B) <i>ABI5</i>, C) <i>AIA</i>, D) <i>AIL</i>, E) <i>RAB18</i>, and F) <i>RD29B</i>. For each gene, the expression level in Col-0 was defined as 1. Data represent means and standard deviation of three replicates.</p

<i>NF-YB</i> overexpression results in late germination.

<p>A–B) <i>nf-yb2</i>, <i>nf-yb3</i> and <i>nf-yb2/b3</i> double mutants on B5 and B5+1 µM ABA media. C–D) <i>p35S::NF-YB2</i> and <i>p35S::NF-YB3</i> on B5 and B5+1 uM ABA media. Germination data is compilation of two experiments (total of n = 6 replicates per genotype) using independent sets of matched seeds. Each replicate contained at least 30 seeds. A Fisher’s Exact Test was performed for both mutants (no difference) and overexpression lines at 84 hrs post-incubation. Both overexpression lines were significantly different (p<0.01) from parental Col-0. Separate, independent <i>NF-YB</i> overexpression lines had similar results.</p

<i>NF-YC</i> are strongly expressed in embryos and the endosperm 24 hours post incubation in light.

<p>A,G) Col-0, B,H) NF-YB2, C,I) NF-YB3, D,J) NF-YC3 E,K) NF-YC4 F,L) NF-YC9. Scale bar in (A) equals 200 µm.</p

<i>NF-YB2-EDLL</i>, but not <i>NF-YB2</i>^<i>E65R</i><i>-EDLL</i>, rescues late flowering in an <i>FT</i>-dependent manner.

<p>Flowering time quantification for 15–20 randomly selected T1 plants of <i>p35S</i>:<i>NF-YB2</i>, <i>p35S</i>:<i>NF-YB2-EDLL</i>, and <i>p35S</i>:<i>NF-YB2</i>^<i>E65R</i><i>-EDLL</i> in A) Col-0 B) <i>co-2</i> C) <i>b2b3</i> D) short days E) <i>ft-10</i>. Asterisks represent significant differences derived from one-way ANOVA (P < 0.05) followed by Bonferroni’s multiple comparison tests (_*** P < 0.001; * P < 0.05).</p