32 research outputs found
From Model Compounds to Proteins: A Volumetric Study of the Stabilizing Action of Glycine Betaine
Folding Thermodynamics of the Hybrid-1 Type Intramolecular Human Telomeric GQuadruplex
Guanine-rich DNA sequences that may form
G-quadruplexes are located in strategic DNA loci with
the ability to regulate biological events. G-quadruplexes
have been under intensive scrutiny owing to their potential
to serve as novel drug targets in emerging anticancer
strategies. Thermodynamic characterization of
G-quadruplexes is an important and necessary step in
developing predictive algorithms for evaluating the conformational
preferences of G-rich sequences in the presence
or the absence of their complementary C-rich
strands. We use a combination of spectroscopic, calorimetric,
and volumetric techniques to characterize the
folding/unfolding transitions of the 26-meric human telomeric
sequence d[A3G3(T2AG3)3A2]. In the presence of
K1 ions, the latter adopts the hybrid-1 G-quadruplex
conformation, a tightly packed structure with an unusually
small number of solvent-exposed atomic groups. The
K1-induced folding of the G-quadruplex at room temperature
is a slow process that involves significant accumulation
of an intermediate at the early stages of the
transition. The G-quadruplex state of the oligomeric
sequence is characterized by a larger volume and compressibility
and a smaller expansibility than the coil state.
These results are in qualitative agreement with each other
all suggesting significant dehydration to accompany the
G-quadruplex formation. Based on our volume data,
432619 water molecules become released to the bulk
upon the G-quadruplex formation. This large number is
consistent with a picture in which DNA dehydration is
not limited to water molecules in direct contact with the
regions that become buried but involves a general
decrease in solute–solvent interactions all over the surface
of the folded structure. VC 2013 Wiley Periodicals, Inc.
Biopolymers 101: 216–227, 2014.
Keywords: G-quadruplexes; conformational transitions;
volume; compressibility; expansibilit
Tribute to Kenneth J. Breslauer
It is an exciting experience to serve as guest editor for a Special Issue celebrating the 75th birthday of Professor Kenneth J [...
Ultrasonic and Densimetric Characterizations of the Hydration Properties of Polar Groups in Monosaccharides
Stabilization of G-Quadruplex-Duplex Hybrid Structures Induced by Minor Groove-Binding Drugs
Once it had been realized that G-quadruplexes exist in the cell and are involved in regulation of genomic processes, the quest for ligands recognizing these noncanonical structures was underway. Many organic compounds that tightly associate with G-quadruplexes have been identified. However, the specificity of G-quadruplex-binding ligands towards individual structures remains problematic, as the common recognition element of these ligands is the G-tetrad. In this paper, we focus on G-quadruplex-duplex hybrids (QDH) containing a hairpin duplex incorporated as a stem-loop into the G-quadruplex core. The presence of a stem-loop renders QDH amenable to sequence-specific recognition by duplex-binding drugs. Should the thermodynamic crosstalk between the stem-loop and the tetraplex core be sufficiently strong, the drug binding to the loop would lead to the stabilization of the entire structure. We studied the stabilizing influence of the minor groove-binders netropsin and Hoechst 33258 on a family of QDH structures, as well as a G-quadruplex and a hairpin modeling the G-quadruplex core and the stem-loop of the QDH’s. We found that the binding of either drug results in an enhancement of the thermal stability of all DNA structures, as expressed by increases in the melting temperature, TM. Analysis of the hierarchical order of increases in TM revealed that the drug-induced stabilization arises from drug binding to the G-quadruplex domain of a QDH and the stem-loop, if the latter contains an all-AT binding site. This result attests to the thermodynamic crosstalk between the stem-loop and the tetraplex core of a QDH. Given the existing library of minor groove-binding drugs recognizing mixed A·T and G·C DNA sequences, our results point to an untapped avenue for sequence-specific recognition of QDH structures in vitro and, possibly, in vivo; thereby, opening the way for selective stabilization of four-stranded DNA structures at predetermined genomic loci, with implications for the control of genomic events