Ultralight vector dark matter search using data from the KAGRA O3GK run

Among the various candidates for dark matter (DM), ultralight vector DM can be probed by laser interferometric gravitational wave detectors through the measurement of oscillating length changes in the arm cavities. In this context, KAGRA has a unique feature due to differing compositions of its mirrors, enhancing the signal of vector DM in the length change in the auxiliary channels. Here we present the result of a search for U(1)B−L gauge boson DM using the KAGRA data from auxiliary length channels during the first joint observation run together with GEO600. By applying our search pipeline, which takes into account the stochastic nature of ultralight DM, upper bounds on the coupling strength between the U(1)B−L gauge boson and ordinary matter are obtained for a range of DM masses. While our constraints are less stringent than those derived from previous experiments, this study demonstrates the applicability of our method to the lower-mass vector DM search, which is made difficult in this measurement by the short observation time compared to the auto-correlation time scale of DM

Purification and characterization of a gelatinolytic serine protease from the seeds of ash gourd <em>Benincasa hispida</em> (Thunb.) Cogn.

77-87In Ayurveda, Benincasa hispida (Thunb.) Cogn. (Ash gourd) was recommended for management of diabetes, peptic ulcer, and other diseases. This plant is rich in proteolytic enzymes and proteases have wide application in food and laundry industry. Therefore, the search for new potential plant proteases continues. A soluble gelatinolytic plant serine protease (AG2) had been purified from the seeds of Benincasa hispida. The molecular mass of the monomer was estimated to be about 11 kDa by SDS-PAGE and 11211.1 Da by MALDI-TOF. The protease activity was strongly inhibited by PMSF only but not at all by soyabean trypsin inhibitor and resists autodigestion. Thus AG2 belongs to subtilisin family. The optimum pH and temperature are 10.0 and 30°C respectively. This protease was quite stable in presence of a cationic surfactant, an oxidizing agent and in basic pH medium. The protease AG2 can hydrolyze casein, azoalbumin and TAME but it was inert towards BAPNA. The kinetic parameters Km and Vmax were 0.117 ± 0.00067 mM and 470.592 ± 0.631 unit mg-1 min-1 respectively using casein as substrate. The CD spectrum showed it as a typical α/β class of protease. The N-terminal sequence of first 17 amino acid residues (MQQFFNEPSSLLIVVVR) is unique in nature

Proceedings of International Web Conference in Civil Engineering for a Sustainable Planet

This proceeding contains articles of the various research ideas of the academic community and practitioners accepted at the "International Web Conference in Civil Engineering for a Sustainable Planet (ICCESP 2021)". ICCESP 2021 is being Organized by the Habilete Learning Solutions, Kollam in Collaboration with American Society of Civil Engineers (ASCE), TKM College of Engineering, Kollam, and Baselios Mathews II College of Engineering, Kollam, Kerala, India. Conference Title: International Web Conference in Civil Engineering for a Sustainable PlanetConference Acronym: ICCESP 2021Conference Date: 05–06 March 2021Conference Location: Online (Virtual Mode)Conference Organizer: Habilete Learning Solutions, Kollam, Kerala, IndiaCollaborators: American Society of Civil Engineers (ASCE), TKM College of Engineering, Kollam, and Baselios Mathews II College of Engineering, Kollam, Kerala, India