High frequency of M. leprae DNA detection in asymptomatic household contacts

Submitted by Sandra Infurna ([email protected]) on 2018-09-28T14:16:37Z No. of bitstreams: 1 euzenirn_sarno_etal_2018.pdf: 520056 bytes, checksum: bdf1ca4f6d571e04b1ac64a89e23c703 (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2018-09-28T14:38:42Z (GMT) No. of bitstreams: 1 euzenirn_sarno_etal_2018.pdf: 520056 bytes, checksum: bdf1ca4f6d571e04b1ac64a89e23c703 (MD5)Made available in DSpace on 2018-09-28T14:38:42Z (GMT). No. of bitstreams: 1 euzenirn_sarno_etal_2018.pdf: 520056 bytes, checksum: bdf1ca4f6d571e04b1ac64a89e23c703 (MD5) Previous issue date: 2018Universidade Vale do Rio Doce. Núcleo de Pesquisa em Imunologia. Governador Valadares, MG, Brasil / Universidade Federal de Juiz de Fora. Programa de Pós Graduação em Ciências Biológicas (Imunologia e DIP/Genética e Biotecnologia). Juiz de Fora, MG, Brasi.Universidade Vale do Rio Doce. Núcleo de Pesquisa em Imunologia. Governador Valadares, MG, Brasil.Universidade Vale do Rio Doce. Núcleo de Pesquisa em Imunologia. Governador Valadares, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Universidade Vale do Rio Doce. Núcleo de Pesquisa em Imunologia. Governador Valadares, MG, Brasil / Universidade Federal de Juiz de Fora. Campus Governador Valadares. Governador Valadares, MG, Brasil.Universidade Vale do Rio Doce. Núcleo de Pesquisa em Imunologia. Governador Valadares, MG, Brasil / Universidade Federal de Juiz de Fora. Programa de Pós Graduação em Ciências Biológicas (Imunologia e DIP/Genética e Biotecnologia). Juiz de Fora, MG, Brasi.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Universidade Federal de Juiz de Fora; Programa de Pós Graduação em Ciências Biológicas (Imunologia e DIP/Genética e Biotecnologia; Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Campus Governador Valadares. Governador Valadares, MG, Brasil / Universidade Federal de Juiz de Fora; Programa de Pós Graduação em Ciências Biológicas (Imunologia e DIP/Genética e Biotecnologia; Juiz de Fora, MG, Brasil.Characterization of the Mycobacterium leprae genome has made possible the development of Polymerase Chain Reaction (PCR) systems that can amplify different genomic regions. Increased reliability and technical efficiency of quantitative PCR (qPCR) makes it a promising tool for early diagnosis of leprosy. Index cases that are multibacillary spread the bacillus silently, even before they are clinically diagnosed. Early detection and treatment could prevent transmission in endemic areas

High frequency of M. leprae DNA detection in asymptomatic household contacts

Abstract Background Characterization of the Mycobacterium leprae genome has made possible the development of Polymerase Chain Reaction (PCR) systems that can amplify different genomic regions. Increased reliability and technical efficiency of quantitative PCR (qPCR) makes it a promising tool for early diagnosis of leprosy. Index cases that are multibacillary spread the bacillus silently, even before they are clinically diagnosed. Early detection and treatment could prevent transmission in endemic areas. Methods In this study, the qPCR technique is used to detect DNA of M. leprae in samples of slit skin smears (SSS) of the ear lobe and blood of leprosy patients and their asymptomatic household contacts residing in Governador Valadares, MG, Brazil, a hyperendemic area for leprosy. A total of 164 subjects participated in the study: 43 index cases, 113 household contacts, and, as negative controls, 8 individuals who reported no contact with patients nor history of leprosy in the family. The qPCR was performed to amplify 16S rRNA fragments and was specifically designed for M. leprae. Results Of asymptomatic household contacts, 23.89% showed bacillary DNA by qPCR in samples of SSS and blood. Also, 48.84% of patients diagnosed with leprosy were positive for qPCR while the bacillary load was positive in only 30.23% of patients. It is important to note that most patients were already receiving treatment when the collection of biological material for qPCR was performed. The level of bacillary DNA from household contacts was similar to the DNA levels detected in the group of paucibacillary patients. Conclusion Considering that household contacts comprise a recognizable group of individuals with a high risk of disease, as they live in close proximity to a source of infection, qPCR can be used to estimate the risk of progress towards leprosy among household contacts and as a routine screening method for a chemoprophylactic protocol