Anthelmintic activity of Cymbopogon martinii, Cymbopogon schoenanthus and Mentha piperita essential oils evaluated in four different in vitro tests

AbstractAnthelmintic resistance is a worldwide concern in small ruminant industry and new plant-derived compounds are being studied for their potential use against gastrointestinal nematodes. Mentha piperita, Cymbopogon martinii and Cymbopogon schoenanthus essential oils were evaluated against developmental stages of trichostrongylids from sheep naturally infected (95% Haemonchus contortus and 5% Trichostrogylus spp.) through the egg hatch assay (EHA), larval development assay (LDA), larval feeding inhibition assay (LFIA), and the larval exsheathment assay (LEA). The major constituent of the essential oils, quantified by gas chromatography for M. piperita oil was menthol (42.5%), while for C. martinii and C. schoenanthus the main component was geraniol (81.4% and 62.5%, respectively). In all in vitro tests C. schoenanthus essential oil had the best activity against ovine trichostrongylids followed by C. martini, while M. piperita presented the least activity. Cymbopogon schoenanthus essential oil had LC50 value of 0.045mg/ml in EHA, 0.063mg/ml in LDA, 0.009mg/ml in LFIA, and 24.66mg/ml in LEA. The anthelmintic activity of essential oils followed the same pattern in all in vitro tests, suggesting C. schoenanthus essential oil could be an interesting candidate for nematode control, although in vivo studies are necessary to validate the anthelmintic properties of this oil

Resistance of cattle of various genetic groups to the tick Rhipicephalus microplus and the relationship with coat traits

AbstractThis study evaluated the resistance of cattle of different genetic groups to the tick Rhipicephalus microplus and the relationship with traits of the animals’ hair and coat. Cows of the Senepol×Nelore (SN), Angus×Nelore (AN) and Nelore (NX) genetic groups were submitted to four consecutive artificial infestations, at 14-day intervals, each one with approximately 20,000 tick larvae placed on the animals’ lumbar region. From the 19th to 23rd day of each infestation five counts of the number of ticks were performed on each animal's left body side. The tick count data (TTC) were transformed into log10 (n+1), and also into percentage of return (PR), where n is the total number of ticks counted at each infestation. Hair samples were collected 24h after the last infestation with flat-nosed pliers. Measures of the average hair length (HL), coat thickness (CT), number of hairs per cm2 (NHCM2) and weight of the samples (SW) were obtained. Pearson's correlation coefficients were calculated within genetic group to measure association between PR and the hair and coat data. There was a significant difference among genetic groups for the number of ticks, with the AN group having higher counts than the SN and NX groups. For the hair and coat traits, the NX and SN groups had lower values of HL and SW than did the AN group. The SN genetic group had lower NHCM2 counts than the NX and AN groups. There were positive correlations between TTC and CT (P<0.05) and SW (P<0.05) in the SN group. No significant correlation was found for the AN genetic group (P>0.05)