Cysticercosis in free-ranging agoutis (Dasyprocta leporina) in the Eastern Brazilian Amazon

Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Patologia. São Paulo, SP, Brasil / Universidade Paulista. Instituto de Ciências da Saúde. Campinas, SP, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Centro Nacional de Primatas. Ananindeua, PA, Brasil.Universidade de São Paulo. Instituto de Medicina Tropical. Departamento de Gastroenterologia. São Paulo, SP, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.The study describes the occurrence of cysticercosis in liver of 22 wild agoutis (Dasyprocta leporina) in the Brazilian Amazon. The phylogenetic analysis and microscopic characteristics of metacestodes in liver tissue sections, associated with the geographic distribution of the intermediate hosts indicated that a possibly novel Taenia sp. metacestode caused the parasitism. Additionally, two cases of hepatic co-infection by Taenia sp., Calodium sp. and Echinococcus oligarthra were also observed among the analyzed animals. The results point to the need for a better understanding of hepatotropic parasites among wild rodents in the Brazilian Amazon

A paruterinid metacestode in the liver of a Neotropical bat (Molossus molossus)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / University of São Paulo. Faculty of Veterinary Medicine and Animal Science. Department of Pathology. São Paulo, SP, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.University of São Paulo. Faculty of Veterinary Medicine and Animal Science. Department of Pathology. São Paulo, SP, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.We describe a case of microscopic liver lesion caused by larval cestode in a frugivorous bat (Molossus molossus) from the Western Brazilian Amazon. Histopathological analysis of liver indicated the occurrence of metacestode associated with multifocal histiocytic response and the phylogenetic analysis of Cox-I and 18S rDNA genes indicated that the parasite belonged to the family Paruterinidae. This is the first identification of cestodes of the family Paruterinidae in bats and may suggest a broad range of paruterinid tapeworm hosts to be investigated in the Brazilian Amazon

Orthohepadnavirus infection in a neotropical bat (Platyrrhinus lineatus)

Instituto Nacional de Ciencia e Tecnologia para Febres Hemorrágicas Virais/Instituto Evandro Chagas/Secretaria de Vigilância em Saude/Ministerio da Saude (ICAAF 240/2008/FAPESPA/INCT); Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ) (155809/2014-8; 141398/2015-9; 573.739/2008-0)University of São Paulo. School of Veterinary Medicine and Animal Science. Department of Pathology. São Paulo, SP, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.University of São Paulo. School of Veterinary Medicine and Animal Science. Department of Pathology. São Paulo, SP, Brazil.University of São Paulo. School of Veterinary Medicine and Animal Science. Department of Pathology. São Paulo, SP, Brazil.Pasteur Institute. São Paulo, SP, Brazil.Pasteur Institute. São Paulo, SP, Brazil.University of São Paulo. School of Medicine. Department of Gastroenterology. São Paulo, SP, Brazil / University of São Paulo. School of Medicine. Institute of Tropical Medicine. São Paulo, SP, Brazil.University of São Paulo. School of Medicine. Department of Gastroenterology. São Paulo, SP, Brazil / University of São Paulo. School of Medicine. Institute of Tropical Medicine. São Paulo, SP, Brazil.University of São Paulo. School of Veterinary Medicine and Animal Science. Department of Pathology. São Paulo, SP, Brazil.Hepatitis B virus (HBV) is the prototype of the Orthohepadnavirus genus and represents an important cause of chronic hepatitis, liver cirrhosis, and hepatic cancer in humans worldwide. To verify the occurrence and genetic variability of orthohepadnavirus among neotropical bats, we tested 81 liver samples of New World bats from Sa similar to o Paulo State, Southeastern Brazil, collected during 2012. PCR, sequencing, and phylogenetic analysis of Surface/ Polymerase and Core viral genes confirmed the occurrence of the first isolate of bat orthohepadnavirus detected in South America. These results may contribute to subsequent studies of the origin, variability, host species, and evolution of bat orthohepadnaviruses in South America

Marcadores sorológicos para hepatite A entre mamíferos silvestres cativos e de vida livre do Estado do Pará, Brasil

Federal Rural University of the Amazon. Pós-Graduate Program Animal Healt and Production in the Amazon. Belém, PA, Brazil.University Paulista. São Paulo, SP, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Federal Rural University of Amazon. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Programa de Pós-Graduação em Virologia. Ananindeua, PA, Brasil.Federal Rural University of the Amazon. Departament Animal Pathology. Belém, PA, Brazil.The hepatitis A virus (HAV, Hepatovirus A) in humans has a relevant impact on public health, especially in geographic regions with poor basic sanitation, such as the Brazilian Amazon. Isolates related to hepatoviruses have been described in non human primates, chiropterans, marsupials, rodents, marmots, shrews, and terrestrial hedgehogs. However, the circulation of these viruses in the Amazonian mammal fauna remains unexplored. This study aimed to evaluate the prevalence of antibodies against this hepatovirus in captive and free-living wild mammals belonging to the orders Didelphimorphia, Primates, Carnivora, and Artiodactyla. Serum samples from 71 animals, from three municipalities in the State of Pará (Belém, Santarém, and CapitãoPoço) were tested for total anti-HAV and anti-HAV IgM through enzyme-linked immunosorbent assay (ELISA). Total anti-HAV antibodies were detected in 29.5% (21/71) of non-human primates, 8.4% (6/71) of carnivores, and 5.6% (4/71) of didelphos. All tayassuidos 0% (0/2) were seronegative. Anti-HAV IgM antibodies were not detected in any of the samples tested. The highest total anti-HAV seropositivity in the municipalities studied was in Santarém with 54.24% (12/22), followed by Capitão Poço with 50% (15/30), and then Belém with 21.05% (4/19). Among the seropositive animals, animals kept in exposure cages showed 43.9% (18/41), quarantined animals with 60% (9/15), and free-living animals with 26.6% (4/15). The serological results indicated a profile of previous exposure to hepatovirus among these animals. Therefore, additional studies to characterize HAV-related viruses in captive and free living wild animals need to be conducted to better understand the impact of the circulation of this virus on human and animal health