Slow sedimentation and deformability of charged lipid vesicles

The study of vesicles in suspension is important to understand the complicated dynamics exhibited by cells in vivo and in vitro. We developed a computer simulation based on the boundary-integral method to model the three dimensional gravity-driven sedimentation of charged vesicles towards a flat surface. The membrane mechanical behavior was modeled using the Helfrich Hamiltonian and near incompressibility of the membrane was enforced via a model which accounts for the thermal fluctuations of the membrane. The simulations were verified and compared to experimental data obtained using suspended vesicles labelled with a fluorescent probe, which allows visualization using fluorescence microscopy and confers the membrane with a negative surface charge. The electrostatic interaction between the vesicle and the surface was modeled using the linear Derjaguin approximation for a low ionic concentration solution. The sedimentation rate as a function of the distance of the vesicle to the surface was determined both experimentally and from the computer simulations. The gap between the vesicle and the surface, as well as the shape of the vesicle at equilibrium were also studied. It was determined that inclusion of the electrostatic interaction is fundamental to accurately predict the sedimentation rate as the vesicle approaches the surface and the size of the gap at equilibrium, we also observed that the presence of charge in the membrane increases its rigidity

El colesterol - una molécula con mala reputación

Es natural que el colesterol no sea visto con vuenos ojos por la población en general, y se podría decir, sin miedo a equivocarse, que esta molécula se ha ganado una muy mala reputación. Sin embargo, en este artículo intentamos presentar el colesterol desde otra pespectiva, la de una molécula exquisitamente diseñada con una función fisiolólgica sencial: regular la organización y complejidad de las membranas celulares, fomentando la diversidad funcional de los diferentes sistemas celulares del organism