Regression de métastases de mélanome après thérapeutique immunologique

In one patient, the appearance of superficial skin metastases of melanoma was not modified by scarification with vaccinia vaccine. Regression was observed after topical application of 2,4-dinitrochloro-benzene which resulted in complete clearing of the tegument which had persisted for over 2 years. Histological examination of some of the regressing lesions revealed infiltration by mononuclear cells and eosinophils as well as the disappearance of the neoplastic melanocytes.</jats:p

RGDS and DGEA-induced [Ca2+]i signalling in human dermal fibroblasts

AbstractA pulse of short peptides, RGDS and DGEA in the millimolar range, immediately elicits in normal human fibroblasts a transient increase of intracellular Ca2+ ([Ca2+]i). In the present study, we show that this [Ca2+]i occurs in an increasing number of cells as a function of peptides concentration. It is specific of each peptide and inhibited at saturating concentration of the peptide in the culture medium. The [Ca2+]i transient depends on signalling pathways slightly different for DGEA and RGDS involving tyrosine kinase(s) and phosphatase(s), phospholipase C, production of inositol-trisphosphate and release of Ca2+ from the cellular stores. GFOGER, the classical collagen binding peptide of α1- α2- and α11-β1 integrins, in triple helical or denatured form, does not produce any Ca2+ signal. The [Ca2+]i signalling induced by RGDS and DGEA is inhibited by antibodies against β1 integrin subunit while that mediated by RGDS is also inhibited by antibodies against the α3 integrin. Delay in the acquisition of responsiveness is observed during cell adhesion and spreading on a coat of fibronectin for RGDS or collagen for DGEA or on a coat of the specific integrin-inhibiting antibodies but not by seeding cells on GFOGER or laminin-5. This delay is suppressed specifically by collagenase acting on the collagen coat or trypsin on the fibronectin coat. Our results suggest that free integrins and associated focal complexes generate a Ca2+ signal upon recognition of DGEA and RGDS by different cellular pathways

Exploration et thérapeutique des lymphomes cutanés à cellules T

We report the observation of 16 cases of cutaneous T cell lymphoma consisting of 11 cases with mycosis fungoides and 5 cases with high-grade malignant lymphomas. A standardized clinical evaluation is proposed for patients with low-grade malignant lymphomas for whom we favor the use of non-aggressive therapeutic regimens. High-grade lymphomas need a more complete systemic exploration. Treatment of cutaneous T cell lymphomas should be started with the least aggressive antineoplastic therapy.</jats:p