Odds ratios (95% confidence interval (CI) for the association between cardiovascular events and three anthropometric indexes as criteria for MetS definition.

<p>Odds ratios (95% confidence interval (CI) for the association between cardiovascular events and three anthropometric indexes as criteria for MetS definition.</p

Sensitivity and specificity for the identification of clustering of two or more risk factors of MetS in both genders.

<p>Sensitivity and specificity for the identification of clustering of two or more risk factors of MetS in both genders.</p

ROC curves for the different anthropometric indices.

<p>Cut-off values were assessed by the Youden index.</p

Prevalence (%) of metabolic syndrome based on anthropometric variables.

<p>Prevalence (%) of metabolic syndrome based on anthropometric variables.</p

Hepatic paramters in apoE^-/- mice fed a high-fat diet (control) or a diet supplemented with 0.3% TTA for 12 weeks.

<p>In liver extracts, fatty acids were measured using gas-liquid chromotography, enzyme activities were measured spectrophotometrically and lipid deposition was assessed using Red Oil staining. <b>A</b>) Tetradecylthioacetic aicd and <b>B</b>) TTA:1n-8, <b>C</b>) Carnitine palmitoyltransferase 1a activity, <b>D</b>) carnitine palmitoyltransferase 2 <b>E</b>) 3-hydroxy-3-methylglutaryl coenzyme A synthase activity <b>F</b>) Citrate synthase activity, <b>G</b>) Acyl-coenzyme A oxidase activity, <b>H</b>) Lipids droplets and <b>I</b>) Triacylglycerols. Bars represent means ± SD in 4-6 mice for each diet. Unpaired <i>t</i>-test was used to detect statistical significance and results significantly different from control are indicated *P<0.05, **P<0.01, ***P<0.001.</p

Levels of mRNA expression in aorta and inflammatory mediators in plasma in apoE^−/− mice fed a high-fat diet (control) or a diet with 5% SPH for 12 weeks.

<p>(A) The gene expressions of the inflammatory markers <i>Icam1</i>, <i>Vcam1</i>, <i>Nos2</i> and <i>Mcp1</i> were measured in pooled aortic arch from six mice. Inflammatory markers in blood samples collected at day 77 of treatment were analysed (B) IL-1β, (C) IL-6, (D) IL-10, (E) TNF-α, (F) GM-CSF and bars represent means ± SD of 4 pooled samples of 3 mice for each diet. Unpaired <i>t</i>-test was used to assess statistical significance and results significantly different from control are indicated (*P<0.05, **P<0.01).</p

Plasma lipids in apoE^−/− mice fed a high-fat casein diet (control) or a high-fat diet with 5% SPH after 77 days of dietary treatment.

1<p>mmol/L.</p><p>Data are shown as mean ± SD (<i>n</i> = 4).</p><p>Abbreviations: NEFA, non-esterified fatty acid; SPH, salmon protein hydrolysate; TAG, triacylglycerol.</p><p>*P<0.05 vs. control.</p

Atherosclerotic plaque level in apoE^−/− mice fed a high-fat diet (control) or a diet with 5% SPH.

<p>After 12 weeks of dietary treatment, whole aorta was collected and <i>en-face</i> analysis was performed to quantify aortic surface covered by atherosclerotic plaques. Bars represent means ± SD of 12 mice for each diet. Unpaired <i>t</i>-test was used to detect statistical significance (*P<0.05).</p

Plasma concentration of inflammatory mediators in apoE^-/- mice fed a high-fat diet (control) or a diet supplemented with 0.3% TTA for 11 weeks.

<p>Blood samples collected at day 77 of treatment were analysed <b>A</b>) IL-1α, <b>B</b>) IL-6, <b>C</b>) IL-17, <b>D</b>) TNF-α and <b>E</b>) IFN-γ. Bars represent means ± SD for 4 mice for each diet. Unpaired <i>t</i>-test was used to assess statistical significance and results significantly different from control are indicated *P<0.05, **P<0.01, ***P<0.001.</p

Plasma lipid concentrations of apoE^-/- mice fed a high-fat diet (control) or a diet supplemented with 0.3% TTA.

<p>After 28, 56 and 77 days of diet induction, blood was collected, EDTA plasma was isolated and plasma lipids were determined by enzymatic assay. <b>A</b>) Triacylglycerols, <b>B</b>) Non-esterified fatty acids, <b>C</b>) Cholesterol, <b>D</b>) Free cholesterol, <b>E</b>) High-density lipoprotein cholesterol and <b>F</b>) Low-density lipoprotein cholesterol. Bars represent means ± SD for 4 mice for each diet. Unpaired <i>t</i>-test was used to detect statistical significance and results significantly different from control are indicated *P<0.05, **P<0.01, ***P<0.001.</p