Gene-gene interaction results for susceptibility to classic Papillary Thyroid Carcinoma.

<p>SH- SNPHarvester; MSH- MegaSNPHunter; Light grey shading indicates interactions selected by each of the algorithms; dark grey green shading indicates the interaction fulfilling the established criteria to pass to stage 2 (replication) (SNP pair selected by at least three methods);</p>*<p>associated P-value; NA- not available (all genotypes combinations classified as neutral).</p

Relative expression levels of STK17B in Pax8-silenced cells.

<p>PCCl3 cells were transiently (A) or stably (B) silenced for the transcription factor Pax8 (siPax8). As a control, wild type or siScramble transfected cells were used. The expression levels were assessed by means of qRT-PCR (A, upper panel) or western blot (A, lower panel, and B).</p

Association with risk of follicular cell-derived thyroid cancer for 9 candidate variants in the discovery and replication stages.

<p>Abbreviations: MAF = minor allele frequency; OR = odds ratio; CI = confidence interval; ESE = Exonic Splicing Enhancers; PTC = Papillary Thyroid Carcinoma; cPTC = classic PTC; fvPTC = follicular variant of PTC; FTC = Follicular Thyroid Carcinoma. The table is sorted by disease subtype and, within each group, by <i>P</i>-value.</p>a<p>Major/minor allele (in controls);</p>b<p>OR and CI were obtained using homozygotes for the most frequent allele in controls as the reference group;</p>c<p><i>P</i>-values are derived from Wald statistics;</p>d<p>Results adjusted for age and gender;</p>e<p>Results adjusted for age, gender and country.</p

Epistatic model for SNPs in <i>PAX8</i> and <i>STK17B</i> and genotype frequencies for cPTC-cases and controls.

<p>Relative frequencies of the nine genotype combinations of the replicated interaction (<i>PAX8</i>-<i>STK17B</i>) are shown for cases and controls (red and blue columns, respectively). The cell containing the high-risk genotype combination is highlighted in light red, those with low-risk combinations in light blue, and those with neutral combinations are uncoloured. Figure1a - based on the discovery stage (series I); <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0074765#pone-0074765-g001" target="_blank">Figure 1b</a> - based on the replication stage (series II and III); <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0074765#pone-0074765-g001" target="_blank">Figure 1c</a> – based on both stages combined (series I, II and III).</p

STK17B and PAX8 mRNA levels in thyroid tumoral cell lines.

<p>The mRNA expression levels of both genes were determined by qRT-PCR and are represented as relative units. The name of the cell line and its origin (follicular, papillary or anaplastic) is indicated.</p