HPLC assay for meprednisone in tablets

A high performance liquid chromatographic (HPLC) method is presented for the determination of meprednisone in the presence of its degradation products using a reverse phase C18 column at ambient temperature with mobile phase consisting of acetonitrile:0.04M dibasic sodium phosphate, pH adjusted to 7.0 (45:65, v/v). The flow rate was 1.3mL/min. Quantitation was achieved with UV detection at 245nm based on peak area. The method was developed and validated for the determination of meprednisone in tablets. The proposed method was validated for selectivity, linearity, accuracy, and robustness. The method was found to be suitable for the quality control of meprednisone in tablets as well as the stability indicating studies.Fil: Ceresole, Rita. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Control de Calidad de Medicamentos; ArgentinaFil: Rosasco, María Ana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Control de Calidad de Medicamentos; ArgentinaFil: Segall, Adriana Ines. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Control de Calidad de Medicamentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentin

A Stability Indicating HPLC Method for the Determination of Benzophenone-3 and Avobenzone in Cosmetic Formulations

An accurate, simple, and reproducible liquid chromatographic method was developed and validated for the determination of benzophenone-3 and avobenzone in a cosmetic formulation. The analyses were performed at room temperature on a reverse-phase C18 column (Inerstil ODS-3) (250 4.6 mm, 5 lm). The mobile phase, which consisted of methanol:water (95:5) and pH 3.2 adjusted with 85% of phosphoric acid, was pumped at a constant flow rate of 1 mL=min. Detection was performed on a UV detector at 315 nm. The method was validated in terms of linearity, precision, accuracy, and specificity by forced decomposition of benzophenone-3 and avobenzone using acid, base, water, hydrogen peroxide, heat, and light. The response of was linear in the range 0.08 to 0.24 mg=mL and 0.04 to 0.12 mg=mL for benzophenone-3 (r2 ¼ 0.9984), and avobenzone (r2 ¼ 0.9925), respectively. The relative standard deviation values for intra- and inter-day precision studies were 0.81 and 0.91 for benzophenone-3 and 1.57 and 1.13 for avobenzone. Recoveries ranged between 99.58 and 101.39 for benzophenone-3 and 98.63 and 102.05 for avobenzone.Fil: Ceresole, Rita. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina;Fil: Han, Yong K.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina;Fil: Simionato, Laura Daniela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina;Fil: Segall, Adriana Ines. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina

Drug-excipient compatibility studies in binary mixtures of Avobenzone

During preformulation studies of cosmetic/pharmaceutical products, thermal analysis techniques are very useful to detect physical or chemical incompatibilities between the active and the excipients of interest that might interfere with safety and/or effi cacy of the fi nal product. Differential scanning calorimetry (DSC) was used as a screening technique for assessing the compatibility of avobenzone with some currently used cosmetic excipients. In the fi rst phase of the study, DSC was used as a tool to detect any interaction. Based on the DSC results alone, cetearyl alcohol, isopropyl myristate, propylparaben, diethylhexyl syringylidene malonate, caprylic capric triglyceride, butylated hydroxytoluene (BHT), glycerin, cetearyl alcohol/ceteareth 20, cetearyl alcohol/sodium lauryl sulfate/sodium cetearyl sulfate, and paraffi num liquidum exhibit interaction with avobenzone. Stressed binary mixtures (stored at 50°C for 15 days) of avobenzone and excipients were evaluated by high-performance liquid chromatography. Binary mixtures were further investigated by infrared (IR) spectroscopy. Based on DSC, isothermal stress testing, and fourier transform infrared results; avobenzone is incompatible with caprylic capric triglyceride, propylparaben, and BHT.Fil: Ceresole, Rita. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina;Fil: Han, Yong K.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina;Fil: Rosasco, María Ana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina;Fil: Orelli, Liliana Raquel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Orgánica; Argentina;Fil: Segall, Adriana Ines. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina

Evaluation of thermal stability and sun protector factor values in vitro in O/W emulsions containing Benzophenone-3 and Avobenzone

The purpose of this work is to study the thermal stability of benzophenone-3 and avobenzone in the presence and in the absence of TiO2, in O/W emulsions for sunscreens products. The emulsions were stored in glass containers at 40 °C and 75 % RH and at room temperature. Concomitantly the Sun Protector Factor (SPF) value was evaluated in vitro. These formulations seem to be more stable in the presence of diethylhexyl syringylidene malonate and titanium dioxide and silica. Avobenzone and benzophenone-3 seem to be less stable in the presence of caprylic capric triglyceride though this excipient increases the SPF in vitro value. Thus, the aim of this study was to evaluate the thermal stability by HPLC and the efficacy of formulations containing chemical filters, a physical filter and an antioxidant measuring the SPF in vitro value.Fil: Ceresole, Rita. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Asero, Marta Delia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Han, Yong Ki. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rosasco, María Ana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Segall, Adriana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin