Anti-VEGF-A antibody does not recognize VEGF-A : Incubation with VEGF-A antibody

This antibody recognizes the VEGF-A monomer (22.5 kDa) and dimer (45 kDa) in control and glaucomatous retinas (first two lanes). VEGF-A antibody does not recognize 25 ng, 100 ng, or 250 ng of VEGF-A recombinant protein in the same membrane (last three lanes). : Incubation of the same membrane with the anti-VEGF antibody after stripping. The anti-VEGF antibody recognizes VEGF-A recombinant protein at all concentrations.<p><b>Copyright information:</b></p><p>Taken from "Levels of vascular endothelial growth factor-A (VEGF-A) are elevated in experimental glaucoma"</p><p></p><p>Molecular Vision 2008;14():1517-1524.</p><p>Published online 18 Aug 2008</p><p>PMCID:PMC2518529.</p><p></p

Immunohistochemical analysis of VEGF-A expression in the glaucomatous retina after five days of elevated IOP

-: Negative control. Some non-specific staining of blood vessels in the RGC and the INL was observed. -: VEGF-A staining of the normal retina (n=4). VEGF-A was present in the RGC and the INL. -: VEGF-A staining of the glaucomatous retina (n=4). Staining was detected in the RGC and INL. VEGF-A levels did not differ between the normal and glaucomatous retinas.<p><b>Copyright information:</b></p><p>Taken from "Levels of vascular endothelial growth factor-A (VEGF-A) are elevated in experimental glaucoma"</p><p></p><p>Molecular Vision 2008;14():1517-1524.</p><p>Published online 18 Aug 2008</p><p>PMCID:PMC2518529.</p><p></p