Analysis of the Interaction between Pisum sativum L. and Rhizobium laguerreae Strains Nodulating This Legume in Northwest Spain

[EN] Abstract: Pisum sativum L. (pea) is one of the most cultivated grain legumes in European countries due to the high protein content of its seeds. Nevertheless, the rhizobial microsymbionts of this legume have been scarcely studied in these countries. In this work, we analyzed the rhizobial strains nodulating the pea in a region from Northwestern Spain, where this legume is widely cultivated. The isolated strains were genetically diverse, and the phylogenetic analysis of core and symbiotic genes showed that these strains belong to different clusters related to R. laguerreae sv. viciae. Representative strains of these clusters were able to produce cellulose and cellulases, which are two key molecules in the legume infection process. They formed biofilms and produced acyl-homoserine lactones (AHLs), which are involved in the quorum sensing regulation process. They also exhibited several plant growth promotion mechanisms, including phosphate solubilization, siderophore, and indole acetic acid production and symbiotic atmospheric nitrogen fixation. All strains showed high symbiotic efficiency on pea plants, indicating that strains of R. laguerreae sv. viciae are promising candidates for the biofertilization of this legume worldwide

Efecto de un inóculo micorrízico en dos especies forestales de Nicaragua

Tesis (MSc en Biología de la Conservación)-Universidad Nacional Autónoma de Nicaragua, LeónUNAN-Leó

Aislamiento de "Micromonospora" de nódulos de leguminosas tropicales y análisis de su interés como promotor del crecimiento vegetal

En el presente trabajo se plantea el aislamiento, caracterización e identificación de cepas de Micromonospora aisladas de nódulos fijadores de nitrógeno de las leguminosas tropicales Phaseolus vulgaris y Arachis hypogeae recogidas en Nicaragua y que son de importancia agrícola en toda América Latina. Posteriormente se realizarán experimentos de reinoculación en planta con el fin de ampliar nuestros conocimientos sobre el papel que pueden desempeñar estos microorganismos en la nodulación y fijación de nitrógeno de las plantas hospedadoras