More than an Uterotonic Agent: Oxytocin Prevents Peritoneal Adhesion

WOS: 000374312100011Prevention of postoperative adhesions (PPA) has become an important issue. The aim is to investigate the effect of Oxytocin (OT) on PPAs. A total of thirty female Wistar-albino rats were randomly divided into three groups (10 rats/group). The cecal peritone of Group I rats (controls) were scraped, to trigger adhesion formation, and no treatment were given. After cecal scrubbing, 1 mL saline solution was applied to each rat in Group II (i.p. saline treated group) and 80 IU/kg of OT (Pituisan (R), Ege Vet, Turkey) to Group III (i.p. OT treated group) intraperitoneally. All animals were sacrificed 10 days after surgery and adhesions graded in terms of severity and histopathologic characteristics. The median scores for the extent, severity, and degree of adhesions in Group I and Group II were statistically significant and considerably higher than those scores for Group III (P<0.001). The inflammation, neovascularization, and fibrosis scores for Group III were statistically significant and considerably lower than those scores for Groups I and II (P<0.001, P<0.001 and P=0.002 respectively). OT, significantly prevented adhesion formation improving wound healing possibly by suppressing adhesion formation with anti-inflammatory and antioxidant properties. OT may be useful in the prevention of PPA in humans

Uterotonik Bir Ajandan Fazlası: Oksitosin Postoperatif Adezyonları Önlüyor

Postoperatif adezyonların (PPA) önlenmesi önemli bir konu olmuştur. Bu çalışmada amaç Oksitosinin (OT) PPA üzerindeki etkisini araştırmaktır. Toplam 30 Wistar-albino dişi rat üç eşit gruba ayrıldı (10 rat/grup). Grup I ratlarda (kontrol) sekum peritonu adezyon oluşturmak amacıyla sıyrıldı ve hiçbir tedavi verilmedi. Grup II'ye (i.p. salin tedavi grubu) sekum sıyrılmasından sonra intraperitoneal 1 ml saline ve Grup III (i.p. OT tedavi grubu)'e 80I U/kg (Pituisan®, Ege Vet, Turkey) OT uygulandı. Bütün hayvanlar cerrahiden 10 gün sonra öldürüldü ve adezyonlar şiddeti ve histolojik özellikleri açısından derecelendirildi. Grup I ve II'deki adezyonların yaygınlık, şiddet ve derecelerinin medyan skorlarının Grup III'dekilere göre istatistiksel olarak arttığı görüldü (P<0.001). Grup III'deki inflamasyon, neovaskülarizasyon ve fibrozis skorları Grup I ve II'deki skorlara göre istatistiksel olarak anlamlı ve belirgin ölçüde düşük bulundu (P<0.001, P<0.001 and P=0.002 respectively). Oksitosin, anti-enflamatuarve anti- oksidan özellikleri ile adezyon oluşum sürecindeki basamakları baskılayarak, yara iyileşmesini düzenleyerek ratlarda adezyon oluşumunu ciddi ölçüde engellemiştir. OT insanlarda PPA önlenmesinde faydalı olabilir.Prevention of postoperative adhesions (PPA) has become an important issue. The aim is to investigate the effect of Oxytocin (OT) on PPAs. A total of thirty female Wistar-albino rats were randomly divided into three groups (10 rats/group). The cecal peritone of Group I rats (controls) were scraped, to trigger adhesion formation, and no treatment were given. After cecal scrubbing, 1 mL saline solution was applied to each rat in Group II (i.p. saline treated group) and 80 IU/kg of OT (Pituisan&reg;, Ege Vet, Turkey) to Group III (i.p. OT treated group) intraperitoneally. All animals were sacrificed 10 days after surgery and adhesions graded in terms of severity and histopathologic characteristics. The median scores for the extent, severity, and degree of adhesions in Group I and Group II were statistically significant and considerably higher than those scores for Group III (P<0.001). The inflammation, neovascularization, and fibrosis scores for Group III were statistically significant and considerably lower than those scores for Groups I and II (P<0.001, P<0.001 and P=0.002 respectively). OT, significantly prevented adhesion formation improving wound healing possibly by suppressing adhesion formation with anti-inflammatory and antioxidant properties. OT may be useful in the prevention of PPA in humans