qRT-PCR expression profiles of <i>A. tequilana</i> fructosyltransferase and invertase genes in different plant tissues and developmental stages.

<p>A. <i>Atq1-SST-2,</i> B. <i>AtqVinv-1</i>, C. <i>Atq6G-FFT-1</i>, D. <i>AtqCwinv-1.</i> S-stem, BL-Base of leaf and ML-Mid-leaf of 1 and 3, year old plants (vegetative stage) and 5 and 7 year old plants (Post-reproductive stage). Photographic examples of tissues sampled are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035878#pone.0035878.s001" target="_blank">Figure S1</a>.</p

Schematic representation of the genomic structures of <i>A. tequilana</i> Fructosyltransferases and Invertases.

<p>Introns are represented by lines and exons with solid boxes. Exons are distinguished by Roman numerals from left to right. Only <i>AtqCwinv-1</i> shows a distinct pattern of exon/intron number and organization. Drawings are to scale and scale bar represents 250 bp.</p

Unrooted tree of selected fructosyltransferases, fructan exohydrolases and invertases from monocotyledonous and dicotyledonous plants.

<p>A: branch containing cell wall invertases and FEHs, B: branch containing vacuolar invertases and fructosyltransferases, b1/b2-monocotyledons, b3-dicotyledons. <i>A. tequilana</i> fructosyltransferases and invertases are indicated with a diamond. Accession numbers are provided in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035878#pone.0035878.s005" target="_blank">Table S1</a>.</p

Alignment of deduced amino acid sequences of fructosyltransferases and Invertases of <i>A. tequilana</i>.

<p>Asterisks, colons and periods indicate identical residues, conserved substitutions, and semi-conserved substitutions, respectively. Putative initiation points of the large and small subunits are arrowed. Potential glycosylation sites are underlined. The β-fructosidase motif, RDP motif and the cysteine catalytic site are boxed. A sucrose-donor substrate motif is shown as a stippled box. Predicted leader sequence cleavage points are shaded in grey. Differences between <i>Atq1-SST-1</i> and SSTAg are shown in italics.</p

Activity of recombinant 6G-FFT-1 protein.

<p>HPAEC chromatograms of the products obtained by the reactions supplied with: A. 100 mM sucrose. B. 50 mM 1-kestose C. both 100 mM sucrose and 50 mM 1-kestose D-amplification of the chromatogram in C. Abbreviations G-glucose, F-fructose, S-sucrose, 1-K-1-kestose, Neo-Neokestose, Nys-nystose, 4c-1^F,6^G-Di-β-D-fructofuranosylsucrose. Unidentified products are indicated by asterisks.</p

Activities of recombinant AtqCwinv-1 and Atq1-SST-2 proteins.

<p>A. Time-course of reaction products when 100 mM sucrose is supplied as substrate for AtqCinv-1. B. HPAEC chromatograms of the products when 100 mM 1-kestose was supplied as substrate for AtqCinv-1. C. Time course of reaction products of Atq1-SST2 when 100 mM sucrose is supplied as substrate and D. HPAEC chromatograms of the products of the Atq1-SST2 reactions when 100 mM sucrose was supplied as substrate. G-glucose, F-fructose, S-sucrose, 1-K-1-kestose, Neo-Neokestose, Nys-nystose.</p

Probability of an allele being undetected for different numbers of fractions of a 60-allele sample, under several dilution thresholds and population allele frequencies.

<p>Probability of an allele being undetected for different numbers of fractions of a 60-allele sample, under several dilution thresholds and population allele frequencies.</p

Plots of information parameters for several bulk sizes, based on estimated allele frequencies in maize germplasm sources.

<p>Plots of information parameters for several bulk sizes, based on estimated allele frequencies in maize germplasm sources.</p

Average values for information parameters in bulks of 10 to 100 SSR alleles in maize.

<p>Average values for information parameters in bulks of 10 to 100 SSR alleles in maize.</p

Informativeness of SSRs.

<p>Distribution of informativeness in 30 SSR loci measured in bits (a). Association between SSR marker informativeness and number of alleles per locus (b).</p