Microsatellites

This file includes the raw allele scores for eight microsatellites collected from 149 samples of the Scinax perpusillus species group (S. perpusillus, S. peixotoi, and S. faivovichi) at several localities in the Brazilian Atlantic Forest

Idiograms with DAPI-CMA₃ bands present in the species of <i>Boana</i> and <i>Hyloscirtus</i> studied in this paper.

<p>Scale indicates percentage relative size of chromosome pairs.</p

C banding pattern in karyotypes of the <i>Boana faber</i> and <i>B</i>. <i>pulchella</i> groups.

<p><b>A.</b><i>B</i>. <i>faber</i>. <b>B.</b><i>B</i>. <i>cipoensis</i>. <b>C.</b><i>B</i>. <i>curupi</i>. <b>D.</b><i>B</i>. <i>stellae</i>. <b>E.</b><i>B</i>. <i>albonigra</i>. <b>F.</b><i>B</i>. <i>riojana</i>. <b>G.</b><i>B</i>. <i>marianitae</i>. <b>H.</b><i>B</i>. <i>bischoffi</i>. <b>I.</b><i>B</i>. <i>cordobae</i>. <b>J.</b><i>B</i>. <i>pulchella</i>. <b>K.</b><i>B</i>. <i>caingua</i>.</p

Studied species and the cytogenetic techniques applied for each one.

<p>Studied species and the cytogenetic techniques applied for each one.</p

Karyotypes of three species of <i>Hyloscirtus</i>.

<p><i>Hyloscirtus alytolylax</i> (left), <i>H</i>. <i>palmeri</i> (center), and <i>H</i>. <i>larinopygion</i> (right). <b>A, B.</b> Giemsa staining. <b>C, D.</b> C-bands. <b>E, F.</b> CMA₃. <b>G, H.</b> DAPI. Squares show NOR-bearing chromosome pairs as stained by the silver impregnation technique (<b>I</b>), and with FISH using a 18S DNA probe (<b>II</b>).</p

Optimization of the basic number (right) and the position of NORs (left) in Cophomantini on a condensed phylogenetic hypothesis resulting from the analyses of Faivovich et al. [21] and Duellman et al. [7].

<p>Optimization of the basic number (right) and the position of NORs (left) in Cophomantini on a condensed phylogenetic hypothesis resulting from the analyses of Faivovich et al. [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0192861#pone.0192861.ref021" target="_blank">21</a>] and Duellman et al. [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0192861#pone.0192861.ref007" target="_blank">7</a>].</p

Triploid specimen of <i>Boana pulchella</i> (LGE 11504).

<p><b>A</b>. Giemsa stained karyotype. <b>B.</b> C banded pattern. <b>C.</b> Meiotic cell in metaphase I stage. <b>D.</b> Normal spermatid (<b>I</b>) and abnormal microspermatid (<b>II</b>).</p

Estimated conductance maps for <i>Dendropsophus minuts</i> complex.

<p>Conductance maps constructed with the program Circuitscape. Conductances were estimated according to a spacial distribution modeling for the <i>D. minutus</i> complex projected to four different climatic scenarios. Conductance maps were averaged and thresholded to show stable dispersal corridors (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0103958#s2" target="_blank">methods</a> for details).</p

<i>Dendropsophus minutus</i> tree with lineage distribution part 3.

<p>50% Maximum Clade Credibility tree and distribution maps of mtDNA lineages 29–43. Asterisks represent nodes with probability equals to 1. Probabilities lower than 0.9 are not shown.</p

Comparative range size of lineages.

<p>Range size of lineages within the <i>Dendropsophus minutus</i> complex. Most lineages appear to be microendemic and are only recorded from one or two localities while eight lineages have ranges larger than 100,000 km².</p