Relationship between Oral Anaerobic Bacteria and Otitis Media with Effusion

Objective: In this study hypothesing the translocation of oral bacteria from oropharynx into the middle ear cavity may be involved in the pathogenesis of otitis media with effusion (OME), we aimed to investigate the presence and similarity of Fusobacterium nucleatum and Treponema denticola in saliva, nasopharyngeal secretion and the middle ear effusion samples from the children with OME

Laryngoscopic Examination During the COVID-19 Pandemic: Turkish Voice Speech and Swallowing Disorders Society and Turkish Professional Voice Society Recommendations

COVID-19 is highly transmissible and spreads rapidly in the population. This increases the occupational risk for health care workers. In otolaryngology clinic practice, patients with upper respiratory tract infection symptoms are common. Also, routine head and neck examinations such as oral cavity examination, nasal/nasopharyngeal examination, or video laryngostroboscopic evaluation are highly risky because of the aerosol formation. To emphasize this issue, two leading otolaryngology organizations in Turkey; 'Voice Speech and Swallowing Disorders Society', and 'Professional Voice Society' gathered a task force. This task force aimed to prepare a consensus report that would provide practical recommendations of the safety measurements during routine clinical care of laryngology patients. To fulfill this, universal aim, on the 2nd and 9th of May 2020, two web-based meetings were conducted by 20 expert physicians. This eighteen items list was prepared as an output

Paradoxical Vocal Fold Motion Disorder: Etiology, Clinical Presentation and Diagnosis

Paradoxical vocal fold motion disorder (PVFMD) is a disease characterized by intermittent adduction of the vocal folds during inspiration and/or expiration, causing dyspnea and sensation of throat tightness. Usually there are intermittent symptoms of shortness of breath and inspiratory stridor that may lead patient to emergency units. The disease is frequently misdiagnosed and many patients are treated wrongly. Some neurologic alterations are accused but the etiology is still unclear. The diagnostic work-up should include a detailed history and physical examination, pulmonary function testing, measures of oxygenation and flexible laryngoscopy. Asthma, laryngopharyngeal reflux, some air irritants and some psychologic conditions should be ruled out in differential diagnosis

REAL-TIME POLYMERASE CHAIN REACTION VERSUS CONVENTIONAL PCR: A COMPARISON BETWEEN TWO METHODS FOR THE DETECTION OF FUSOBACTERIUM NUCLEATUM IN SALIVA, NASOPHARYNGEAL SECRETION AND MIDDLE EAR EFFUSION SAMPLES

Oral and middle ear infections are biofilm related infections. Fusobacterium nucleatum is considered to be a key species in dental biofilm formation. The aim of this study was to compare conventional and real-time PCR, for the detection of F. nucleatum in saliva, nasopharyngeal secretion and middle ear effusion samples. Sixty samples were collected from 20 patients with otitis media with effusion. Species-specific 165 rRNA primers were used for conventional PCR and species-specific primers and TaqMan probes were used for real-time PCR analysis. Real-time PCR had a better performance than conventional PCR in detecting bacteria in the analyzed samples. The tested real-time PCR assay can identify) and identify) F nucleatum in biofilm samples with a high degree of sensitivity when compared with conventional PCR techniques