S2 Fig -

(A) Genomic DNA sequence of rat HID-1. The predicted Cas9 cleavage site is indicated. (B) Indels (shown in red) detected in INS-1 HID-1 KO cells. (C) Indels (shown in red) detected in PC12 HID-1 KO cells. (D-E) HID-1 KO PC12 and INS-1 immunostained for endogenous HID-1. Scale bars indicate 10μm. (TIF)</p

Artificial tethering of the HID-1 C-terminus domain to the TGN is sufficient to rescue the HID-1 KO phenotype.

(A-C) HID-1 KO PC12 cells were transiently co-transfected with GFP-TGN38, GFP-TGN38-HID-1-C-terminus or HID-1-GFP and NPY-sfCherry3. (A) Anti-GFP immunoblot showing expression level of the fusion proteins. (B-C) Cells were washed and incubated in Tyrode’s buffer containing 2.5 mM K+ (basal) or 90 mM K+ (stimulated) for 30 min at 37°C. The amount of cellular and secreted NPY-sfCherry3 was determined using a plate reader (Tecan). *, p D) HID-1 KO PC12 cells were transiently co-transfected with GFP, GFP-TGN38-HID-1-C-terminus, immunostained for SgII and the amount of fluorescence in GFP positive cells was quantified and normalized to WT PC12 cells. ***, p<0.001 relative to HID-1-KO by one-way ANOVA (n = 226 cells for WT, n = 217 cells for KO, n = 186 cells for GFP, n = 185 cells for GFP-TGN38-Cterm from three independent experiments). The bar graphs indicate mean ± s.e.</p

HID-1 myristoylation is not required for function.

(A-C) HID-1 KO PC12 cells transduced with indicated HID-1-HA lentivirus were washed and incubated for 30 minutes in Tyrode’s solution containing 2.5 mM K+ (basal) or 90 mM K+ (stimulated). Cellular and secreted SgII were measured by quantitative fluorescent immunoblotting (A), with the secreted SgII normalized to tubulin and expressed as percent of basal secretion in the KO (B), and the cellular SgII normalized to tubulin (C). *, p < 0.05 relative to HID-1 KO by one-way ANOVA (n = 4). The bar graphs indicate mean ± s.e.</p

Protein sequence alignment of HID-1 C-terminus (103 amino acids) for the indicated species and for the human mutation.

Protein sequence alignment of HID-1 C-terminus (103 amino acids) for the indicated species and for the human mutation.</p

S1 Fig -

(A) Protein sequence alignment of HID-1 N-terminus. (B) PC12 cells were transfected with the indicated GFP constructs. Cells were treated or not with saponin were fixed and immunostained for TGN38. Scale bars indicate 10μm. (TIF)</p

HID-1 N-terminus mediates membrane binding but is not strictly required for TGN localization.

(A) INS-1 cells were co-transfected with sialyltransferase-RFP657 and indicated GFP constructs. Cells treated or not with saponin were fixed and imaged. Quantification of the number of GFP positive cells was performed using flow cytometry and data represent the percentage changes in the number of GFP positive cells after saponin treatment compared to untreated cells (>3000 cells per experiment). ***, pB) INS-1 cells were transfected with WT, G2A or Δ22 HID-1-HA and treated or not with saponin before fixation and immunostained for HA and TGN38. TGN-localized and total HA immunoreactivity was quantified and expressed as a ratio. *, p C-E) INS-1 HID-1 KO cells stably expressing WT or G2A HID-1-GFP were transiently transfected with sialyltransferase-RFP657 and analyzed by fluorescence loss in photobleaching. The white boxes represent the defined photobleaching region of interest (ROI). Arrows designate the TGN (determined by co-transfected sialyltransferase-RFP657—not shown). Time (0:00) indicates the timeframe immediately after the first photobleaching pulse. Quantification of the change in fluorescence at the TGN is shown in (D) and of the initial decay slope in (E). **, p<0.01; WT n = 4, G2A n = 3 independent sialyltransferase-RFP657 transfections. Scale bar indicates 10μm. The bar graphs indicate mean ± s.e.</p

S1 Raw images -

Large dense core vesicles (LDCVs) mediate the regulated release of neuropeptides and peptide hormones. HID-1 is a trans-Golgi network (TGN) localized peripheral membrane protein contributing to LDCV formation. There is no information about HID-1 structure or domain architecture, and thus it remains unknown how HID-1 binds to the TGN and performs its function. We report that the N-terminus of HID-1 mediates membrane binding through a myristoyl group with a polybasic amino acid patch but lacks specificity for the TGN. In addition, we show that the C-terminus serves as the functional domain. Indeed, this isolated domain, when tethered to the TGN, can rescue the neuroendocrine secretion and sorting defects observed in HID-1 KO cells. Finally, we report that a point mutation within that domain, identified in patients with endocrine and neurological deficits, leads to loss of function.</div

List of constructs and primers.

Large dense core vesicles (LDCVs) mediate the regulated release of neuropeptides and peptide hormones. HID-1 is a trans-Golgi network (TGN) localized peripheral membrane protein contributing to LDCV formation. There is no information about HID-1 structure or domain architecture, and thus it remains unknown how HID-1 binds to the TGN and performs its function. We report that the N-terminus of HID-1 mediates membrane binding through a myristoyl group with a polybasic amino acid patch but lacks specificity for the TGN. In addition, we show that the C-terminus serves as the functional domain. Indeed, this isolated domain, when tethered to the TGN, can rescue the neuroendocrine secretion and sorting defects observed in HID-1 KO cells. Finally, we report that a point mutation within that domain, identified in patients with endocrine and neurological deficits, leads to loss of function.</div

Homozygous mutation within HID-1 C-terminus identified in human patients fail to rescue regulated secretion.

(A) HID-1 KO PC12 cells stably expressing WT HID-1 or the human mutant were immunostained for HA and TGN38. (B-D) Secretion assays were performed and quantified as described in Fig 2 using HID-1 KO PC12 cells stably expressing WT or HID-1 human mutant. *, p < 0.05 relative to WT by one-way ANOVA (n = 4). The bar graphs indicate mean ± s.e. Scale bar indicates 10μm.</p

The highly conserved C-terminus of HID-1 serves as the functional domain.

(A) Secretion assays were performed using HID-1 KO PC12 cells stably expressing WT HID-1 or indicated HID-1 truncations. Cellular and secreted SgII were measured by quantitative fluorescent immunoblotting as described in Fig 2, with the secreted SgII normalized to actin and expressed as percent of basal secretion in the KO (B), and the cellular SgII normalized to actin (C). *, p D) HID-1 KO INS-1 cells were stably transfected with indicated constructs. Cells were fixed, immunostained for HA and TGN38, and imaged using a spinning disk confocal. Scale bars indicate 10μm.</p