The influence of p02, BE, BB parameters of perinatally obtained cord blood on quantity of obtained cells and focus on the perfect donor criteria

The aim of the study was to analyze the influence of oxygene partial pressure (p02), base excess (BE) and buffer base (BB) parameters of cord blood obtained perinatally on quantity of obtained cells and focus on the perfect donor criteria. The study included 50 pregnant women aged between 18 and 38 years in which spontaneous labors and elective cesarean sections were performed. Umbilical cord blood was collected immediately after the women were delivered of newborns. The cells were analyzed in the Polish Stem Cells Bank in Warsaw. In the study group of patients different stem cells viability levels did not differ significantly in terms of pO2, BB and BE level, however, there was a trend that the higher the viability the lower BE value. The experiment showed also that the cord blood (CB) oxygenation scope is vitally important for the CB cells viability

Maternal predictors and quality of umbilical cord blood units

The aim of the study was to determine the relationship between the maternal age at delivery and selected properties of the cord blood stem cells. The study included 50 pregnant women aged between 18 and 38 years in which spontaneous labors or elective cesarean sections were performed. Umbilical cord blood was collected immediately after the women were delivered of newborns. The samples were analyzed in the Polish Stem Cells Bank in Warsaw. The highest mean WBC level (p < 0.05) was observed in the umbilical blood collected from patients aged 35 years and more. Similarly, the highest mean cell viability was observed in the umbilical cord blood collected from patients aged 35 and more. There were no statistically significant correlations between the CD34+ cells count and mean cell viability in the umbilical cord blood and the maternal age. With the significance level at p < 0.001, the females after spontaneous labor revealed a visibly higher WBC level than patients after a cesarean section. The higher mean WBC concentration (24.95 thousand/μl) was observed in the umbilical cord blood of patients aged 35 and more after spontaneous labors. In the same group, the umbilical cord blood was also characterized by the highest mean cell viability (98.72%). The number of nucleated cells in the umbilical cord blood collected in the perinatal period increases together with the maternal age. In the course of physiological spontaneous labors, the collected umbilical cord blood has more nucleated cells as compared with elective caesarian sections

Wpływ pH i pCO2 krwi pępowinowej uzyskiwanej okołoporodowo na wybrane parametry komórek macierzystych

Objective: The aim of the study was to demonstrate a correlation between pH and pCO2 levels in umbilical cord blood CD34+ cells and their vitality was analyzed. Material and methods: The study included 50 pregnant women after vaginal delivery at term or elective cesarean section. Umbilical cord blood was collected immediately after birth. The probes were analyzed at the Polish Stem Cell Bank in Warsaw. Results: The number of CD34+ cells ranged from 0.1-0.2 in white blood cells count over 12 thousand/ml and pH of >7.3. If pH ranged between 7.35-7.40, the number of CD34+ was 0.3-0.4. The highest number of CD34+ cells was noted for pH of 7.30-7.35 and amounted to 0.4-0.5. Analysis of stem cell vitality showed that the highest level, over 98%, was obtained when pH was 40.0 mmHg. For pCO2Cel pracy: Celem badania było wykazanie korelacji pomiędzy poziomem pH i pCO2 krwi pępowinowej pobieranej od dawcow, a jakością pozyskanego materiału. Dodatkowo postanowiono znaleźć zależność wpływu pH i pCO2 krwi pępowinowej na ilość komorek CD34+, ich żywotność oraz wpływ sposobu zakończenia ciąży na właściwości krwi pępowinowej. Materiał i metody: Do badania zakwalifikowano 50 ciężarnych kobiet, u ktorych wystąpił porod siłami natury lub elektywne cięcie cesarskie w terminie porodu. Krew pępowinowa została pobrana bezpośrednio po urodzeniu noworodka. Probki poddano analizie w Polskim Banku Komorek Macierzystych w Warszawie. Wyniki: Przy liczbie leukocytow powyżej 12 tys./μl i jednoczesnym pH >7,3 ilość CD34+ mieściła się w przedziale 0,1-0,2. W przypadku gdy pH wynosi 7,35 -7,40 zakres wartości CD34+ wyniosł 0,3-0,4. Największą ilość CD34+ uzyskano dla pH 7,30-7,35 i wynosiła ona 0,4-0,5. Badając żywotność komorek macierzystych, jej największy poziom >98% uzyskano gdy pH wyniosło poniżej 7,3 oraz gdy pH było ≥7,4. Badania wykazały, że żywotność komorek macierzystych obniżała się do 97-98% przy pH 7,3-7,4. Zaobserwowano niskie wartości CD34+ (0,01-0,09) przy wartościach pCO2 >40,0 mmHg. Przy pCO