Diffusion characterization of the different biomaterials.

<p>a) Calculated diffusion coefficients. Data points represent the mean ± SEM. **p<0.01, ***p<0.001. n = 3.</p

Calculated Young’s modulus for the different bead compositions.

<p>Data points represent the mean ± SEM. *p<0.05, **p<0.01. n = 10.</p

Bead implantation in mice (zoom 20x).

<p>(a) Ca-alg, (b) Ca-alg mixed with collagen. The beads were implanted during 2 weeks and the tissues were fixed and stained with Hemalun Eosine Safran (HES). B) Beads, C) Inflammatory cells.</p

HepG2/C3A cell viability at day 8 in the different biomaterials using confocal laser scanning.

<p>(a) Alginate, (b) Alginate+PLL and alginate layers, (c) Alginate mixed with collagen, (d) Alginate mixed with collagen+PLL and alginate layers. Cells were stained with Acridine Orange (living cells, in green) and Propidium Iodide (dead cells, in red). The white bars correspond to 50 µm.</p

Beads obtained with the different material conditions.

<p>(a) alginate, (b) alginate with PLL and alginate layers, (c) alginate mixed with collagen, (d) alginate mixed with collagen and PLL and alginate layers. The white bars correspond to 50 µm. (e) Bead diameter evolution during the 10 days of culture.</p

HepG2/C3A cell viability and proliferation.

<p>(a) Cell viability measured by quantification of the released LDH. The cell viability was higher than 80% from the third day of culture. (b) Cell number measured by DNA content. The DNA measurement was normalized with respect to measurement at 1 day post-encapsulation. Data points represent the mean ± SEM. *p<0.05, **p<0.01, ***p<0.001. n = 4.</p

Different types of material tested.

<p>Different types of material tested.</p