CIL:40660

Platinum replica depicting the cytoskeletal organization of dendritic spines in extracted 14 DIV neurons. Mushroom spines associate with dendrites at the base (bottom) and with axons by the head (top). Unlabeled insets show the smaller versions of the corresponding main panels with axons color-coded in purple, dendrites in yellow, and spines in cyan. Thick fibers in neurites represent microtubules. Red asterisks indicate putative PSD fragments. Yellow boxes are enlarged in panels with corresponding numbers. Box 1, interaction of putative PSD (arrow) from the spine head with axonal intermediate filaments (green); actin filaments are shown in cyan and a microtubule in red. Boxes 2 and 3, branched actin filaments (cyan) in the head (2) and at the neck–head junction (3). Insets in panels 2 and 3 show nonpseudocolored regions outlined by yellow boxes. Dashed arrows in panel 3 indicate potential filament breakage. Bars, 0.2 μm. Image corresponds to Figure 2a in Mol Biol Cell. 2010 Jan 1;21(1):165-76. The entire panel (this image) is available as CIL 40660. The uncolorized image is available as CIL 40659. Box 1, 2, and 3 are CIL 40661, 40662, and 40663, respectively

CIL:1594

The purpose of the dataset C. elegans terminal bulb aging is to deduce the age of the nemathode based on images of the pharynx terminal bulb (part of the eating apparatus). Images of C. elegans were taken at different chronological ages. This image is part of the day 0 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH

CIL:1869

The purpose of the dataset C. elegans terminal bulb aging is to deduce the age of the nemathode based on images of the pharynx terminal bulb (part of the eating apparatus). Images of C. elegans were taken at different chronological ages. This image is part of the day 2 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH

CIL:1770

The purpose of the dataset C. elegans terminal bulb aging is to deduce the age of the nemathode based on images of the pharynx terminal bulb (part of the eating apparatus). Images of C. elegans were taken at different chronological ages. This image is part of the day 2 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH

CIL:1137

The purpose of the dataset C. elegans muscle aging is to deduce the age of the nemathode based on images of muscles. Images of C. elegans were taken at different chronological ages. This image is part of the day 4 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH

CIL:1613

The purpose of the dataset C. elegans terminal bulb aging is to deduce the age of the nemathode based on images of the pharynx terminal bulb (part of the eating apparatus). Images of C. elegans were taken at different chronological ages. This image is part of the day 0 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH

CIL:39144

Cells of the symbiotic prochlorophyte Prochloron didemni were separated from its ascidian partner, thylakoids isolated, treated to liberate chlorophyll-containing complexes, and negatively stained preparations observed by transmission EM. The image shows an averaged top view of a chlorophyll-photsystem II super complex with an overlay illustrating the putative locations of the core dimer and surrounding CP43 proteins obtained from x-ray analysis data. The overview shown in CIL 39146 illustrates the range of particle types in the preparation

CIL:39466

Early ascidian (sea squirt) embryos visualized by differential interface contrast (DIC) microscopy. Ascidians are used as a model for developmental research. Their simple embryonic development is rapid and can be easily manipulated. In addition, each embryo contains only a few cells therefore complex cellular processes can be studied while remaining part of an intact embryo. The embryos are also transparent, which is ideal for fluorescent microscopy thus allowing scientists to visualize different developmental stages. A higher magnification image is available as CIL 39471

CIL:28744

One of the principal challenges in counting or segmenting nuclei is dealing with clustered nuclei. To help assess algorithms' performance in this regard, this synthetic image set consists of five subsets with increasing degree of clustering. Five subsets of 20 images each are provided. Each image contains 300 objects, but the objects overlap and cluster with overlap probability ranging from 0.0 to 0.6, and can be found with CIL 27833, 27853, 28754, 28734, and 28714, respectively. This image set has 0.45 probability overlap. The images were generated with the SIMCEP (http://www.cs.tut.fi/sgn/csb/simcep/tool.html) simulating platform for fluorescent cell population images (Lehmussola et al., IEEE T. Med. Imaging, 2007 and Lehmussola et al., P. IEEE, 2008). Ground truth for foreground/background segmentation are available as binary images as the second image in the tiff image stack. Recommended citation We used the Synthetic 1 image set (Ruusuvuori et al., in Proc. of the 16th European Signal Processing Conference (EUSIPCO-2008), 2008), available from the Broad Bioimage Benchmark Collection (www.broad.mit.edu/bbbc)