1 research outputs found
neXtProt: Organizing Protein Knowledge in the Context of Human Proteome Projects
About 5000 (25%) of the ∼20400 human protein-coding
genes
currently lack any experimental evidence at the protein level. For
many others, there is only little information relative to their abundance,
distribution, subcellular localization, interactions, or cellular
functions. The aim of the HUPO Human Proteome Project (HPP, www.thehpp.org) is to collect this information for every human protein. HPP is
based on three major pillars: mass spectrometry (MS), antibody/affinity
capture reagents (Ab), and bioinformatics-driven knowledge base (KB).
To meet this objective, the Chromosome-Centric Human Proteome Project
(C-HPP) proposes to build this catalog chromosome-by-chromosome (www.c-hpp.org) by focusing primarily on proteins that currently
lack MS evidence or Ab detection. These are termed “missing
proteins” by the HPP consortium. The lack of observation of
a protein can be due to various factors including incorrect and incomplete
gene annotation, low or restricted expression, or instability. neXtProt
(www.nextprot.org) is a new web-based knowledge platform
specific for human proteins that aims to complement UniProtKB/Swiss-Prot
(www.uniprot.org) with detailed information obtained from
carefully selected high-throughput experiments on genomic variation,
post-translational modifications, as well as protein expression in
tissues and cells. This article describes how neXtProt contributes
to prioritize C-HPP efforts and integrates C-HPP results with other
research efforts to create a complete human proteome catalog