Genes most highly induced or inhibited in HUVEC cultures 8 h after NiV infection.

<p><b>FC</b>: Fold Change; induction of the level of gene expression in NiV-infected cells in comparison to mock-infected cells as determined by microarray analysis. Only changes higher then 5.7 and lower then −5.4 are presented.</p

Expression of CXCL10 and NiV nucleoprotein (N) genes in organs obtained from NiV-infected hamsters during the course of infection.

<p>Hamsters were sacrificed at different time points after infection and RNA was isolated from different organs and analyzed by RT-qPCR. Grey bars correspond to CXCL10 and black bars to NiV-N, calculated as described in Methods. Significant correlation was found between expression of CXCL10 and expression of NiV N (R² = 0,989, p<0,001, Pearson test).</p

Analysis of expression of interferon-related genes in NiV-infected HUVECs.

<p>A, RT-qPCR analysis of the expression of MXA, OAS1, IFN beta, CXCL11 and CXCL10. B, ELISA analysis of the production of CXCL10 protein in mock-infected (white bars) and NiV-infected (black bars) HUVC cultures. Results are expressed as the average of 2–3 individual experiments and bars represent standard deviation. *p<0.05, **p<0.01, Mann-Whitney U-test.</p

Primary human endothelial cells are highly permissive to NiV-infection.

<p>A, Mock-infected and B, NiV-infected HUVECs (MOI = 1) observed 24 h after infection show extensively developed syncytium formation. C, RT-qPCR analysis of the expression of NiV receptors ephrinB2 (EFNB2) and ephrinB3 (EFNB3) in HUVECs (black bars) and U373 astroglioma cells (white bars). D, Production of RNA specific for NiV genes: nucleoprotein (N), matrix (M), fusion protein (F), glycoprotein (G) and polymerase (L) in HUVECs during infection, determined by RT-qPCR. Fold change is relative to the number of copies of viral mRNAs 8 h or 24 h post infection compared to the number of copies obtained after 1 h of contact with the virus. E, Production of infectious NiV particles during the course of infection; supernatants taken at different time points were titrated on a Vero cell monolayer.</p

Immunohistochemical analysis of CXCL10 production in the brain durng NiV infection.

<p>Non-infected patients (A, ×100) and patients autopsied after lethal acute NiV-infection (B–H) were analysed for the expression of CXCL10 in endothelial cells in cerebral cortex (B, D, ×1000, arrows) and cells with neuronal morphology (E, F). CXCL10 immunoreactivity in perivascular inflammatory cells (C, G, H, ×400).</p

Impact of NiV infection on interferon pathway in HUVEC cells, determined using Ingenuity Pathway Analysis.

<p>NiV-induced upregulated genes are presented grouped, depending the cell compartment in which the corresponding proteins a localized (nucleus, cytoplasm, plasma membrane and extracellular space, for secreted proteins). Level of FC increase is indicated bellow each gene and intensity of red color corresponds to the FC.</p