Diagnostic de la pneumocystose par PCR en temps réel au CHU de Poitiers (comparaison et optimisation des méthodes)

POITIERS-BU Médecine pharmacie (861942103) / SudocSudocFranceF

Diagnostic de la toxoplasmose par PCR en temps réel (mise en place technique et étude de la validation de méthode)

POITIERS-BU Médecine pharmacie (861942103) / SudocSudocFranceF

Etude de l'effet in vitro de deux échinocandines sur la croissance de biofilms de candida albicans en phases intermédiaire et de maturation

POITIERS-BU Médecine pharmacie (861942103) / SudocSudocFranceF

Développement de nouvelles approches in vitro pour l'inhibition des biofilms de candida (recherche de molécules fongiques inhibitrices et mise au point d'un modèle de traitement verrou)

POITIERS-BU Médecine pharmacie (861942103) / SudocSudocFranceF

Candidoses associées aux cathéters

La présence de biofilms fongiques associés aux surfaces de dispositifs médicaux implantés, tels que les cathéters, représente un facteur de risque majeur de développer une candidémie. De plus, les levures de ces biofilms ont une sensibilité diminuée aux antifongiques. Depuis peu, une nouvelle approche thérapeutique a émergé, la lock therapy ou « traitement verrou », qui repose sur l’utilisation de solutions antimicrobiennes à forte concentration, instillées dans la lumière du cathéter et laissées en place pendant 8 à 12 h. Des travaux, réalisés in vitro ou in vivo, ont porté sur l’évaluation de l’efficacité de verrous antifongiques utilisant l’amphotéricine B, un azolé ou des échinocandines. Les résultats, prometteurs pour certaines molécules, permettront de discuter la pertinence de l’utilisation de cette technique

Draft genome sequence of the Rhinocladiella similis clinical isolate CBS 149759

International audienceRhinocladiella similis is a melanized fungi involved in chromoblastomycosis. R. similis genome has never been sequenced, therefore we propose the first draft genome of R. similis

Lichtheimia corymbifera Colonization Leading to Pulmonary Infection Can Be Prevented with Liposomal Amphotericin B in a New Murine Model

International audienceThe incidence of pulmonary mucormycosis is constantly increasing, especially in hematological patients staying in high-efficiency particulate air-filtered rooms. Pulmonary inhalation of spores may occur outside the hospital, leading to invasive disease once patients received chemotherapies. We developed a new pulmonary mucormycosis mouse model mimicking the expected pathophysiology in human to study antifungal drugs. Naive mice were inoculated intratracheally with Lichtheimia corymbifera spores. After 3 days, mice received corticosteroids and cyclophosphamide and secondarily developed the disease, while only 5% of the initial inoculum was present in the lungs at day 3. Lung colonization with L. corymbifera spores in immunocompetent mice can last at least 44 days. Antifungal drug was administered the day of immunosuppression. Injection of a single 15 mg/kg of body weight dose of liposomal amphotericin B significantly improved survival and pulmonary fungal burden compared with controls, whereas 80 mg/kg oral posaconazole did not. These results show that a unique dose of liposomal amphotericin B offers a real potential decolonization treatment to prevent infection in our mouse model of L. corymbifera lung colonization followed by lung infection

Answer to July 2020 Photo Quiz

International audienc

Photo Quiz: Migrating Worm Naturally Exited through the Palate

International audienc

Acanthamoeba castellanii is not be an adequate model to study human adenovirus interactions with macrophagic cells.

Free living amoebae (FLA) including Acanthamoeba castellanii, are protozoa that feed on different microorganisms including viruses. These microorganisms show remarkable similarities with macrophages in cellular structures, physiology or ability to phagocyte preys, and some authors have therefore wondered whether Acanthamoeba and macrophages are evolutionary related. It has been considered that this amoeba may be an in vitro model to investigate relationships between pathogens and macrophagic cells. So, we intended in this study to compare the interactions between a human adenovirus strain and A. castellanii or THP-1 macrophagic cells. The results of molecular and microscopy techniques following co-cultures experiments have shown that the presence of the adenovirus decreased the viability of macrophages, while it has no effect on amoebic viability. On another hand, the viral replication occurred only in macrophages. These results showed that this amoebal model is not relevant to explore the relationships between adenoviruses and macrophages in in vitro experiments