RNA degradation compromises the reliability of microRNA expression profiling

Background: MicroRNAs are small non-coding RNAs that post-transcriptionally regulate gene expression and their expression is frequently altered in human diseases, including cancer. To correlate clinically relevant parameters with microRNA expression, total RNA is frequently prepared from samples that were archived for various time periods in frozen tissue banks but, unfortunately, RNA integrity is not always preserved in these frozen tissues. Here, we investigate whether experimentally induced RNA degradation affects microRNA expression profiles. Results: Tissue samples were maintained on ice for defined time periods prior to total RNA extraction, which resulted in different degrees of RNA degradation. MicroRNA expression was then analyzed by microarray analysis (miCHIP) or microRNA-specific real-time quantitative PCR (miQPCR). Our results demonstrate that the loss of RNA integrity leads to in unpredictability of microRNA expression profiles for both, array-based and miQPCR assays. Conclusion: MicroRNA expression cannot be reliably profiled in degraded total RNA. For the profiling of microRNAs we recommend use of RNA samples with a RNA integrity number equal to or above seven

A Wireless System for Sport Assessment

In the context of sport assessment, the evaluation and monitoring of the referees decisions is of interest for several sports in order to avoid disputes and assist the referees in their activity. Some current solutions are based on video recording, third referee, etc. In this paper, a new wireless wearable system, result of the Italian research project REC-VISIO, is described suitable to assess the referees actions when they are on the move. The system is able to collect the referee staff visual perspective of a sport match, whose subjective is recorded, preprocessed and sent by a wireless network to a sideline workstation for final stabilization. The system architecture design and implementation are described along with the experimentation on the field, considering as use case a football match. The experimentation has shown the successful integration of all the different system components, where the cooperation of sensor-based subsystem, processing unit, and communication subsystem allows to collect and stabilize the video streaming reducing the effects of the movement of the referee, and send in real-time the video content to the sideline workstation without video quality degradation

Photonic Combinatorial Network for Contention Management in 160 Gb/s Interconnection Networks based on All-Optical 2x2 Switching Elements

A modular photonic interconnection network based on a combination of basic 2×2 all-optical nodes including a photonic combinatorial network for the packet contention management is presented. The proposed architecture is synchronous, can handle optical time division multiplexed (OTDM) packets up to 160 Gb/s, exhibits self-routing capability, and very low switching latency. In such a scenario, OTDM has to be preferred to wavelength division multiplexing (WDM) because in the former case, the instantaneous packet power carries the information related to only one bit, making the signal processing based on instantaneous nonlinear interactions between packets and control signals more efficient. Moreover, OTDM can be used in interconnection networks without caring about the propagation impairments because of the very short length (< 100 m) of the links in these networks. For such short-range networks, the packet synchronization can be solved at the network boundary in the electronic domain without the need of complex optical synchronizers. In this paper, we focus on a photonic combinatorial network able to detect the contentions, and to optically drive the contention resolution block and the switching control block. The implementation of the photonic combinatorial network is based on semiconductor devices, which makes the solution very promising in terms of compactness, stability, and power consumption. This implementation represents the first example of complex photonic combinatorial network for ultrafast digital processing. The network performance has been investigated for bit streams at 10 Gb/s in terms of bit error rate (BER) and contrast ratio. Moreover, the suitability of the 2×2 photonic node architecture exploiting the earlier mentioned combinatorial network has been verified at a bit rate up to 160 Gb/s. In this way, the potential of photonic digital processing for the next generation broad band and flexible interconnection networks has been demonstrated

Sublethal necroptosis signaling promotes inflammation and liver cancer

It is currently not well known how necroptosis and necroptosis responses manifest in vivo. Here, we uncovered a molecular switch facilitating reprogramming between two alternative modes of necroptosis signaling in hepatocytes, fundamentally affecting immune responses and hepatocarcinogenesis. Concomitant necrosome and NF-κB activation in hepatocytes, which physiologically express low concentrations of receptor-interacting kinase 3 (RIPK3), did not lead to immediate cell death but forced them into a prolonged "sublethal" state with leaky membranes, functioning as secretory cells that released specific chemokines including CCL20 and MCP-1. This triggered hepatic cell proliferation as well as activation of procarcinogenic monocyte-derived macrophage cell clusters, contributing to hepatocarcinogenesis. In contrast, necrosome activation in hepatocytes with inactive NF-κB-signaling caused an accelerated execution of necroptosis, limiting alarmin release, and thereby preventing inflammation and hepatocarcinogenesis. Consistently, intratumoral NF-κB-necroptosis signatures were associated with poor prognosis in human hepatocarcinogenesis. Therefore, pharmacological reprogramming between these distinct forms of necroptosis may represent a promising strategy against hepatocellular carcinoma

Chromokinesin Xklp1 Contributes to the Regulation of Microtubule Density and Organization during Spindle Assembly

Xklp1 is a chromosome-associated kinesin required for Xenopus early embryonic cell division. Function blocking experiments in Xenopus egg extracts suggested that it is required for spindle assembly. We have reinvestigated Xklp1 function(s) by monitoring spindle assembly and microtubule behavior under a range of Xklp1 concentrations in egg extracts. We found that in the absence of Xklp1, bipolar spindles form with a reduced efficiency and display abnormalities associated with an increased microtubule mass. Likewise, centrosomal asters assembled in Xklp1-depleted extract show an increased microtubule mass. Conversely, addition of recombinant Xklp1 to the extract reduces the microtubule mass associated with spindles and asters. Our data suggest that Xklp1 affects microtubule polymerization during M-phase. We propose that these attributes, combined with Xklp1 plus-end directed motility, contribute to the assembly of a functional bipolar spindle