3 research outputs found
Carotenoid production and gene expression in an astaxanthin‑overproducing Xanthophyllomyces dendrorhous mutant strain
Artículo de publicación ISIThe primary carotenoid synthesized by Xanthophyllomyces
dendrorhous is astaxanthin, which is used
as a feed additive in aquaculture. Cell growth kinetics and
carotenoid production were correlated with the mRNA levels
of the idi, crtE, crtYB, crtI, crtS and crtR genes, and
the changes in gene sequence between the wild-type and a
carotenoid overproducer XR4 mutant strain were identified.
At the late stationary phase, the total carotenoid content in
XR4 was fivefold higher than that of the wild-type strain.
Additionally, the mRNA levels of crtE and crtS increased
during the XR4 growth and were three times higher than
the wild-type strain in the late stationary phase. Moreover,
the nucleotide sequences of crtYB, crtI and crtR exhibited
differences between the strains. Both the higher crtE and
crtS transcript levels and the crtYB, crtI and crtR mutations
can, at least in part, act to up-regulate the carotenoid biosynthesis
pathway in the XR4 strain