Should adjustment for covariates be used in prevalence estimations?

Background Adjustment for covariates (also called auxiliary variables in survey sampling literature) is commonly applied in health surveys to reduce the variances of the prevalence estimators. In theory, adjusted prevalence estimators are more accurate when variance components are known. In practice, variance components needed to achieve the adjustment are unknown and their sample estimators are used instead. The uncertainty introduced by estimating variance components may overshadow the reduction in the variance of the prevalence estimators due to adjustment. We present empirical guidelines indicating when adjusted prevalence estimators should be considered, using gender adjusted and unadjusted smoking prevalence as an illustration. Methods We compare the accuracy of adjusted and unadjusted prevalence estimators via simulation. We simulate simple random samples from hypothetical populations with the proportion of males ranging from 30% to 70%, the smoking prevalence ranging from 15% to 35%, and the ratio of male to female smoking prevalence ranging from 1 to 4. The ranges of gender proportions and smoking prevalences reflect the conditions in 1999–2003 Behavioral Risk Factors Surveillance System (BRFSS) data for Massachusetts. From each population, 10,000 samples are selected and the ratios of the variance of the adjusted prevalence estimators to the variance of the unadjusted (crude) ones are computed and plotted against the proportion of males by population prevalence, as well as by population and sample sizes. The prevalence ratio thresholds, above which adjusted prevalence estimators have smaller variances, are determined graphically. Results In many practical settings, gender adjustment results in less accuracy. Whether or not there is better accuracy with adjustment depends on sample sizes, gender proportions and ratios between male and female prevalences. In populations with equal number of males and females and smoking prevalence of 20%, the adjusted prevalence estimators are more accurate when the ratios of male to female prevalences are above 2.4, 1.8, 1.6, 1.4 and 1.3 for sample sizes of 25, 50, 100, 150 and 200, respectively. Conclusion Adjustment for covariates will not result in more accurate prevalence estimator when ratio of male to female prevalences is close to one, sample size is small and risk factor prevalence is low. For example, when reporting smoking prevalence based on simple random sampling, gender adjustment is recommended only when sample size is greater than 200

Selective immunolesions of CH4 cholinergic neurons do not disrupt spatial memory in rats

Adult male Long-Evans rats were subjected to bilateral lesions of the cholinergic neurons in the nucleus basalis magnocellularis (NBM) by injection of 0.2 or 0.4 microg 192-IgG-saporin in 0.4 microl phosphate-buffered saline. Control rats received an equivalent amount of phosphate-buffered saline. Starting 2 weeks after surgery, all rats were tested for locomotor activity in their home cage, beam-walking performance, T-maze alternation rates (working memory), reference and working memory performance in a water-maze task, and memory capabilities in the eight-arm radial maze task using uninterrupted and interrupted (delay of 2 min, 2 h and 6 h after four arms had been visited) testing procedures. Histochemical analysis showed a significant decrease of acetylcholinesterase (AChE)-positive reaction products (30-66%) in various cortical regions at the 0.2-microg dose. At the dose of 0.4 microg, there was an additional, although weak, damage to the hippocampus (17-30%) and the cingulate cortex (34%). The behavioral results showed only minor impairments in spatial memory tasks, and only during initial phases of the tests (reference memory in the water maze, working memory in the radial maze). The behavioral effects of the dramatic cholinergic lesions do not support the idea of a substantial implication of cholinergic projections from the NBM to the cortex in the memory processes assessed in this study, but they remain congruent with an involvement of these projections in attentional functions

Discutindo a educação ambiental no cotidiano escolar: desenvolvimento de projetos na escola formação inicial e continuada de professores

A presente pesquisa buscou discutir como a Educação Ambiental (EA) vem sendo trabalhada, no Ensino Fundamental e como os docentes desta escola compreendem e vem inserindo a EA no cotidiano escolar., em uma escola estadual do município de Tangará da Serra/MT, Brasil. Para tanto, realizou-se entrevistas com os professores que fazem parte de um projeto interdisciplinar de EA na escola pesquisada. Verificou-se que o projeto da escola não vem conseguindo alcançar os objetivos propostos por: desconhecimento do mesmo, pelos professores; formação deficiente dos professores, não entendimento da EA como processo de ensino-aprendizagem, falta de recursos didáticos, planejamento inadequado das atividades. A partir dessa constatação, procurou-se debater a impossibilidade de tratar do tema fora do trabalho interdisciplinar, bem como, e principalmente, a importância de um estudo mais aprofundado de EA, vinculando teoria e prática, tanto na formação docente, como em projetos escolares, a fim de fugir do tradicional vínculo “EA e ecologia, lixo e horta”.Facultad de Humanidades y Ciencias de la Educació

Geochemical Evolution of Eocene Lakes in the Nevada Hinterland of the North American Cordillera

Abstract Eocene strata of the Elko Formation record lacustrine deposition within the Nevada hinterland of the North American Cordillera. We present a detailed geochemical stratigraphy enabled by high‐sampling‐resolution geochronology from lacus trine limestone and interbedded volcanic rocks of the Elko Formation. Two intervals of lacustrine deposition, an early Eocene “Lake Adobe” of limited aerial extent and a laterally extensive middle Eocene “Lake Elko,” are separated by ∼5 m.y. of apparent unconformity. Sediments deposited in the apparently short‐lived (49.5–48.5 Ma) early Eocene Lake Adobe exhibit high‐amplitude covariation of δ18O, δ13C and 87Sr/86Sr, which suggests a dynamically changing catchment and precipitation regime. Lake Elko formed during the middle Eocene, and its strata record three geochemically distinct phases, indicating it was a single interconnected water body that became increasingly evaporative over time. The lower Elko Formation (44.0–42.5 Ma) was deposited in a freshwater lake. Middle Elko Formation (42.5–41.2 Ma) lithofacies and geochemistry suggest that an increasingly saline and alkaline Lake Elko experienced salinity stratification‐induced hypolimnion disoxia and burial of 12C‐rich organic matter. The upper Elko Formation (41.2–40.5 Ma) records a shallow final phase of Lake Elko that experienced short residence times and a breakdown in stratification. A sharp decline of 87Sr/86Sr in the upper Elko Formation reflects an increasing aerial extent of low‐87Sr/86Sr volcanic deposits from nearby calderas. Middle Eocene strata record ponding of paleodrainage, increasing hydrologic isolation and volcanism, consistent with progressive north to south removal of the Farallon flat slab and/or delamination of the lower lithospheric mantle of the North American plate

Early neural activation during facial affect processing in adolescents with Autism Spectrum Disorder

Impaired social interaction is one of the hallmarks of Autism Spectrum Disorder (ASD). Emotional faces are arguably the most critical visual social stimuli and the ability to perceive, recognize, and interpret emotions is central to social interaction and communication, and subsequently healthy social development. However, our understanding of the neural and cognitive mechanisms underlying emotional face processing in adolescents with ASD is limited. We recruited 48 adolescents, 24 with high functioning ASD and 24 typically developing controls. Participants completed an implicit emotional face processing task in the MEG. We examined spatiotemporal differences in neural activation between the groups during implicit angry and happy face processing. While there were no differences in response latencies between groups across emotions, adolescents with ASD had lower accuracy on the implicit emotional face processing task when the trials included angry faces. MEG data showed atypical neural activity in adolescents with ASD during angry and happy face processing, which included atypical activity in the insula, anterior and posterior cingulate and temporal and orbitofrontal regions. Our findings demonstrate differences in neural activity during happy and angry face processing between adolescents with and without ASD. These differences in activation in social cognitive regions may index the difficulties in face processing and in comprehension of social reward and punishment in the ASD group. Thus, our results suggest that atypical neural activation contributes to impaired affect processing, and thus social cognition, in adolescents with ASD

Variability and Controls on δ18O, d‐excess, and ∆′17O in Southern Peruvian Precipitation

The isotopic composition of precipitation is used to trace water cycling and climate change, but interpretations of the environmental information recorded in central Andean precipitation isotope ratios are hindered by a lack of multi‐year records, poor spatial distribution of observations, and a predominant focus on Rayleigh distillation. To better understand isotopic variability in central Andean precipitation, we present a three‐year record of semimonthly δ18Op and δ2Hp values from 15 stations in southern Peru and triple oxygen isotope data, expressed as ∆′17Op, from 32 precipitation samples. Consistent with previous work, we find that elevation correlates negatively with δ18Op and that seasonal δ18Op variations are related to upstream rainout and local convection. Spatial δ18Op variations and atmospheric back trajectories show that both eastern‐ and western‐derived air masses bring precipitation to southern Peru. Seasonal d‐excessp cycles record moisture recycling and relative humidity at remote moisture sources, and both d‐excessp and ∆′17Op clearly differentiate evaporated and non‐evaporated samples. These results begin to establish the natural range of unevaporated ∆′17Op values in the central Andes and set the foundation for future paleoclimate and paleoaltimetry studies in the region. This study highlights the hydrologic understanding that comes from a combination of δ18Op, d‐excessp, and ∆′17Op data and helps identify the evaporation, recycling, and rainout processes that drive water cycling in the central Andes.Key PointsThe isotopic composition of central Andean precipitation records upstream precipitation, local convection, and remote moisture sourcesPrecipitation on the flank of the western central Andes is sourced from the Pacific Ocean∆’17O can separate evaporated and non‐evaporated samples and provides key baseline information for Andean paleoclimate and paleoaltimetryPeer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/171158/1/2020JD034009-sup-0001-Supporting_Information_SI-S01.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/171158/2/jgrd57452.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/171158/3/jgrd57452_am.pd

Temporal-spatial neural activation patterns linked to perceptual encoding of emotional salience.

It is well known that we continuously filter incoming sensory information, selectively allocating attention to what is important while suppressing distracting or irrelevant information. Yet questions remain about spatiotemporal patterns of neural processes underlying attentional biases toward emotionally significant aspects of the world. One index of affectively biased attention is an emotional variant of an attentional blink (AB) paradigm, which reveals enhanced perceptual encoding for emotionally salient over neutral stimuli under conditions of limited executive attention. The present study took advantage of the high spatial and temporal resolution of magnetoencephalography (MEG) to investigate neural activation related to emotional and neutral targets in an AB task. MEG data were collected while participants performed a rapid stimulus visual presentation task in which two target stimuli were embedded in a stream of distractor words. The first target (T1) was a number and the second (T2) either an emotionally salient or neutral word. Behavioural results replicated previous findings of greater accuracy for emotionally salient than neutral T2 words. MEG source analyses showed that activation in orbitofrontal cortex, characterized by greater power in the theta and alpha bands, and dorsolateral prefrontal activation were associated with successful perceptual encoding of emotionally salient relative to neutral words. These effects were observed between 250 and 550 ms, latencies associated with discrimination of perceived from unperceived stimuli. These data suggest that important nodes of both emotional salience and frontoparietal executive systems are associated with the emotional modulation of the attentional blink