Aislamiento, caracterización y cultivo de células madre de pulpa dental humana y su diferenciación a linaje osteogénico in vitro utilizando factor de crecimiento epidérmico y factor de crecimiento fibroblástico básico.

El factor de crecimiento epidérmico (EGF) y el factor de crecimiento fibroblástico básico (bFGF) juegan un importante papel en la mineralización de la matriz extracelular, un proceso complejo requerido para una apropiada regeneración ósea, uno de los mayores retos en odontología regenerativa. El propósito de este estudio fue evaluar el potencial osteogénico de EGF y bFGF en células madre de pulpa dental (DPSCs). Las DPSCs humanas fueron aisladas utilizando microperlas magnéticas anti-CD105 y caracterizadas por citometría de flujo. Para inducir la diferenciación a osteoblastos, estas células fueron cultivadas en medio osteogénico suplementado con EGF y bFGF a baja concentración. La morfología celular y la expresión de los marcadores de superficie CD146 y CD10 fueron analizadas mediante microscopia de fluorescencia. Para medir la mineralización, se realizó un ensayo de alizarina roja y fueron evaluados los marcadores típicos de fenotipo osteoblástico mediante RT-PCR. El tratamiento con EGF indujo cambios morfológicos y supresión de los marcadores CD146 y CD10. Adicionalmente, las células fueron capaces de producir depósitos de calcio e incrementar la expresión de mRNA para fosfatasa alcalina (ALP) y osteocalcina (OCN) en relación con los grupos control (p < 0.001). Sin embargo, bFGF mostro un efecto inhibitorio. Estos datos sugieren que las DPSCs en combinación con EGF podrían ser una efectiva terapia basada en células madre para ingeniería de tejido óseo en periodoncia e implantología oral

Eficacia antimicrobiana y antibiofilm de un hidrogel cargado con nanopartículas lipofílicas de bismuto sobre bacterias periodonto patógenas.

La enfermedad periodontal es un desorden crónico inflamatorio causado por la colonización de microorganismos en la bolsa periodontal y creciendo dentro de biopelículas. A pesar de ser una infección localizada, existen muy pocas opciones de tratamiento tópicas de tipo no antibiótico para combatir a estos microorganismos

Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro

Introduction: Epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) play an important role in extracellular matrix mineralization, a complex process required for proper bone regeneration, one of the biggest challenges in dentistry. The purpose of this study was to evaluate the osteogenic potential of EGF and bFGF on dental pulp stem cells (DPSCs). Material and methods: Human DPSCs were isolated using CD105 magnetic microbeads and characterized by flow cytometry. To induce osteoblast differentiation, the cells were cultured in osteogenic medium supplemented with EGF or bFGF at a low concentration. Cell morphology and expression of CD146 and CD10 surface markers were analyzed using fluorescence microscopy. To measure mineralization, an alizarin red S assay was performed and typical markers of osteoblastic phenotype were evaluated by RT-PCR. Results: EGF treatment induced morphological changes and suppression of CD146 and CD10 markers. Additionally, the cells were capable of producing calcium deposits and increasing the mRNA expression to alkaline phosphatase (ALP) and osteocalcin (OCN) in relation to control groups (p < 0.001). However, bFGF treatment showed an inhibitory effect. Conclusion: These data suggests that DPSCs in combination with EGF could be an effective stem cell-based therapy for bone tissue engineering applications in periodontics and oral implantology

Antimicrobial Activity of a Cationic Guanidine Compound against Two Pathogenic Oral Bacteria

This study evaluated the potential antimicrobial properties of a polyguanidine (CatDex) on two oral bacteria. Chlorhexidine gluconate 134

Two‐photon detection of organotin Schiff base complexes in cancer cells

The early detection of cancer cells and their visualization before and after surgery are essential for successful pre‐ and post‐operative disease management. Although fluorescence imaging is a sensitive and versatile tool that is finding increasing use in clinical applications, there is a lack of tumour‐targeting fluorophores. We report here a family of fluorescent Schiff base organotin dyes (1: Et2N−L‐SnPh2, 2: Et2N−L‐SnBu2, 3: MeO−L‐SnPh2, 4: MeO−L‐SnBu2, 5: HO−L‐SnPh2, and 6: HO−L‐SnBu2, where L=2‐hydroxybenzylidene‐4‐hydroxybenzhydrazine). In addition to one‐photon‐excited fluorescence, efficient two‐photon excitation was demonstrated in compounds 1–4. Two of the compounds (5 and 6), both with hydroxyl substituents, were capable of selective accumulation in HeLa cells, allowing differentiation from normal cells (periodontal ligament cells). Compounds 1 and 3 showed excellent cancer cell staining (HeLa) using two‐photon bioimaging, which is promising for biomedicine applications

Antimicrobial and antibiofilm activities of MTA supplemented with bismuth lipophilic nanoparticles

The objective of this work was to determine the antimicrobial and antibiofilm properties of mineral trioxide aggregate (MTA) supplemented with bismuth lipophilic nanoparticles (BisBAL NPs). The antimicrobial activity of the composite MTA-BisBAL NPs was determined by the disk diffusion assay, while antibiofilm activity was analyzed by fluorescence microscopy. The cytotoxicity of MTA-BisBAL NPs was determined on human gingival fibroblasts by optical microscopy and crystal violet staining. MTA-BisBAL NPs inhibited the growth of Enterococcus faecalis, Escherichia coli, and Candida albicans and also detached the biofilm of fluorescent E. faecalis after 24 h of treatment. The addition of BisBAL nanoparticles did not significantly modify the physical properties of MTA, and cytotoxicity was not observed when MTA-BisBAL NPs was added on human gingival fibroblasts. Altogether these results suggest that BisBAL nanoparticles provide antimicrobial and antibiofilm activities to MTA while it retained their biophysical properties without cause side effects on human gingival fibroblasts. Keywords: Antimicrobial activity, Antibiofilm activity, Bismuth nanoparticles, Mineral trioxide aggregat

Antimicrobial properties and dental pulp stem cell cytotoxicity using carboxymethyl cellulose-silver nanoparticles deposited on titanium plates

Objective: To evaluate the antimicrobial properties and dental pulp stem cells (DPSCs) cytotoxicity of synthesized carboxymethyl cellulose-silver nanoparticles impregnated on titanium plates. Material and methods: The antibacterial effect of silver nanoparticles in a carboxymethyl cellulose matrix impregnated on titanium plates (Ti-AgNPs) in three concentrations: 16%, 50% and 100% was determined by adding these to bacterial cultures of Streptococcus mutans and Porphyromonas gingivalis. The Ti-AgNPs cytotoxicity on DPSCs was determined using a fluorimetric cytotoxicity assay with 0.12% chlorhexidine as a positive control. Results: Silver nanoparticles in all concentrations were antimicrobial, with concentrations of 50% and 100% being more cytotoxic with 4% cell viability. Silver nanoparticles 16% had a cell viability of 95%, being less cytotoxic than 0.12% chlorhexidine. Conclusions: Silver nanoparticles are a promising structure because of their antimicrobial properties. These have high cell viability at a concentration of 16%, and are less toxic than chlorhexidine