Association of methamidophos and sleep loss on reproductive toxicity of male mice

This study investigated the effects of organophosphate exposure on the male reproductive system of mice submitted to chronic sleep loss condition. Adult Swiss mice were distributed into 4 groups: control; methamidophos (MTP); sleep restriction (SR); and MTP + SR. the dose of methamidophos was 0.002 mg kg(-1) day(-1) (half of the Acceptable Daily Intake). Sleep restriction condition was 21 h day(-1) during 15 days. in relation to control group, MTP treatment induced a significant reduction of 12% on morphologically normal spermatozoa in both MTP and MTP + SR groups. in addition, the absolute and relative weights of the seminal vesicles were decreased (MTP, -34%; MTP + SR, -45%). Epididymal fat was reduced in SR groups (SR, -64%; MTP + SR, -58%). Plasma testosterone levels were significantly decreased in MTP and SR groups, and progesterone levels were increased 8 times in MTP + SR in comparison with the control group. the corticosterone levels were unaffected by MTP or SR conditions. Thus, low dose MTP exposure resulted in deleterious effects on the male reproductive system. Sleep loss associated with MTP potentiated the effect on steroidogenesis, mainly in terms of progesterone levels. (C) 2011 Elsevier B.V. All rights reserved.Associacao Fundo de Incentivo a Psicofarmacologia (AFIP)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FAPEGConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FAPEG/RDPET-MEC/SeSUUniv Fed Goias, Dept Ciencias Fisiol, BR-74001970 Goiania, Go, BrazilUniversidade Federal de São Paulo, Dept Psicobiol, São Paulo, BrazilEMBRAPA Gado Corte, Campo Grande, MS, BrazilUniversidade Federal de São Paulo, Dept Psicobiol, São Paulo, BrazilFAPESP: 98/143030FAPEG: Cham 01/2007FAPEG: Cham 04/2010Web of Scienc