12 research outputs found
Morphological, genetic and molecular desciption of Pristionchus pacificus
Description est donnĂ©e d'une nouvelle espĂšce hermaphrodite de nĂ©matode libre, #Pristionchus pacificus sp. n. (#Neodiplogastridae) qui pourra ĂȘtre utilisĂ©e pour des Ă©tudes de gĂ©nĂ©tique, de dĂ©veloppement et de biologie molĂ©culaire. #P. pacificus sp. n. possĂšde six chromosomes, un cycle de reproduction de trois jours et s'Ă©lĂšve facilement. Quarante-huit mutations morphologiques sont dĂ©crites, ce qui indique l'accessibilitĂ© Ă l'Ă©tude gĂ©nĂ©tique. Des Ă©tudes molĂ©culaires ont dĂ©butĂ© par l'Ă©tablissement de banques d'ADN gĂ©nomique et d'ADNc et par le clonage de l'homologue du gĂšne let-60 ras de #Caenorhabditis elegans. (RĂ©sumĂ© d'auteur
Rootâlesion nematodes of potato: current status of diagnostics, pathogenicity and management
Rootâlesion nematodes of the genus Pratylenchus are migratory endoparasites with worldwide economic impact on several important crops including potato, where certain species like P. penetrans, P. neglectus and P. scribneri reduce the yield and quality of potato tubers. Morphological identification of Pratylenchus spp. is challenging, and recent advancements in molecular techniques provide robust and rapid diagnostics to differentiate species without need of specialist skills. However, the fact that molecular diagnostics are not available for all Pratylenchus species means that there are limitations in worldwide application. In general, rootâlesion nematodes are difficult to manage once introduced into agricultural land and damage can be related to pathogenicity and population densities. In addition, rootâlesion nematodes interact with fungi such as V. dahliae, resulting in disease complexes that enhance the damage inflicted on the potato crop. Management interventions are often focused on limiting nematode reproduction before planting crops and include the application of nematicides, and cultural practices such as crop rotation, cover crops, biofumigation, and biological control. Understanding the limitations of the available crop protection strategies is important and there are many gaps for further study. This review discusses the status of the diagnosis, distribution, pathogenicity and management of the main species of rootâlesion nematodes, reported to infect potatoes worldwide, and highlights areas for potential future research
Improvement of long segment ribosomal PCR amplification for molecular identification of Litylenchus crenatae mccannii associated with beech leaf disease
Morphological, genetic and molecular desciption of Pristionchus pacificus
Description est donnĂ©e d'une nouvelle espĂšce hermaphrodite de nĂ©matode libre, #Pristionchus pacificus sp. n. (#Neodiplogastridae) qui pourra ĂȘtre utilisĂ©e pour des Ă©tudes de gĂ©nĂ©tique, de dĂ©veloppement et de biologie molĂ©culaire. #P. pacificus sp. n. possĂšde six chromosomes, un cycle de reproduction de trois jours et s'Ă©lĂšve facilement. Quarante-huit mutations morphologiques sont dĂ©crites, ce qui indique l'accessibilitĂ© Ă l'Ă©tude gĂ©nĂ©tique. Des Ă©tudes molĂ©culaires ont dĂ©butĂ© par l'Ă©tablissement de banques d'ADN gĂ©nomique et d'ADNc et par le clonage de l'homologue du gĂšne let-60 ras de #Caenorhabditis elegans. (RĂ©sumĂ© d'auteur