163 research outputs found
Normal phase HPLC profiling of the acetylcholinesterase activity in apolar plant extracts
Among nineteen evaluated Clusiaceous species, one stem bark CH2Cl2 crude extract was selected based on a significant inhibition of acetylcholinesterase (AChE) using the micro-dilution Ellman\u27s method [1]. A normal phase HPLC profiling with micro-fractionation of this extract provided discrete fractions every 20 seconds. In order to obtain a comprehensive profiling of AChE activity all microfractions were tested [2] in dilution assay (Ellman) as well as by bioautography (the Fast Blue B salt method). Furthermore the potency of inhibition was evaluated both by keeping the genuine concentration within the extract and after normalisation to a standard concentration level. From the active fractions five pure compounds were isolated and identified. The different methods of sample preparation and biological evaluation associated with normal-phase micro-fractionation of plant extracts are critically discussed
The Carbonyl Group as Homoconjugated Electron Donating Substituent - Abinitio Sto 3g Mo Calculations
Regioselective Additions of Electrophiles to Olefins Remotely Perturbed - the Carbonyl Group as a Homoconjugated Electron Donating Substituent
Abinitio Mo Calculations on the Rearrangements of 7-Oxa-2-Bicyclo[2.2.1]Heptyl Cations - the Facile Migration of Acyl Group in Wagner-Meerwein Rearrangements
A New, Doubly Convergent Synthesis of Anthracyclinones - Diels-Alder Additions to 2,3,5,6-Tetrakis(Methylene)-7-Oxanorbornane
Through-Bond Interactions of Beta-Carbonyl and Beta-Imine Lone Pairs with Cationic 2p Orbital - Quantum Calculations on Bicyclo[2.2.2]Oct-1-Yl Cation and Derivatives
Charge-Transfer Bands of Bicyclic Beta,Gamma-Unsaturated Ketones - Circular-Dichroism of (+)-(1r)-5,6-Dimethylidene-2-Bicyclo[2.2.2]Octanone and (+)-(1r)-2-Bicyclo[2.2.2]Oct-5-Enone
Double-Bond Pyramidalization in Bicyclic Alkanes - Abinitio Mo Calculations on Bicyclo[2.2.1]Hept-2-Ene, Bicyclo[2.1.1]Hex-2-Ene and Bicyclo[3.2.1]Oct-6-Ene Derivatives
Interactions between Nonconjugated Chromophores .28. Regioselective Electrophilic Additions of Bicyclo[2.2.N]Alk-2-Enes Controlled by Remote Epoxide Functions
The Circular-Dichroism of 5,6-Dimethylidene-2-Bicyclo[2.2.N]Alkyl Esters - Chiral Exciton Coupling between Benzoate and Exocyclic S-Cis-Butadiene Chromophores
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