Nativos digitales aprendices de la producción audiovisual

Mucho se afirma de los nativos digitales, pero lo cierto es que nadie nace aprendido. La siguiente comunicación presenta la más reciente experiencia del taller Telekids, una dinámica de aprendizaje destinada a niños entre 8 y 10 años de edad en relación con el universo de la producción audiovisual. La alfabetización mediática tiene ya un largo camino recorrido dilatado en múltiples experiencias, que aunque aisladas, conforman un conglomerado de prácticas dignas de ser reconocidas.Much has been said about the digital natives, but the truth is that nobody is born learned. The following communication has recent experience Telekids workshop, a dynamic of learning aimed at children between 8 and 10 years of age in relation to the world of audiovisual production. Media literacy has a long journey and countless experiences that, although isolated, form large group practices worthy of recognition

Type I Interferon Sensitizes Lymphocytes to Apoptosis and Reduces Resistance to Listeria Infection

Infection with Listeria monocytogenes causes lymphocyte apoptosis that is mediated by the actions of the pore-forming virulence factor listeriolysin O (LLO). Previous work showed that activated lymphocytes were highly sensitive to LLO-induced apoptosis, whereas resting lymphocytes were less susceptible. We now show that mice deficient in the type I interferon (IFN) receptor were more resistant to Listeria infection and had less apoptotic lesions than wild-type counterparts. Furthermore, treatment of resting splenic lymphocytes with recombinant IFN-αA enhanced their susceptibility to LLO-induced apoptosis. Together, these data suggest that type I IFN signaling is detrimental to handling of a bacterial pathogen and may enhance the susceptibility of lymphocytes undergoing apoptosis in response to bacterial pore-forming toxins

Intelligent copolymers based on poly (N-isopropylacrylamide) PNIPAm with potential use in biomedical applications. Part i: PNIPAm functionalization with 3-butenoic acid and piperazine

The synthesis and characterization of the thermosensitive copolymers based on Poly (N-Isopropylacrylamide) (PNIPAm) and 3-butenoic acid and functionalized with piperazine was carried out. The free radical polymerization of the PNIPA copolymer with 3-butenoic acid was performed under microwave radiation. After obtaining this copolymer, the carboxyl groups present in the copolymer chain were activated with 1-ethyl- (3-3-dimethylaminopropyl) carbodiimide in the presence of N-hydroxysuccinimide, improving its reactivity to incorporate the piperazine through its amino group. The characterization consisted: differential scanning calorimetric and ultraviolet-visible spectrophotometry to determine the LCST phase transition temperature, ranging from (30-35)°C. Structurally it was analyzed by infrared spectroscopy. A morphological analysis was performed using scanning electron microscopy, after simulating an injectable process, with the objective to observe internally the porosity and interconnectivity. The biocompatibility was evaluated through hemocompatibility tests and it was observed that the copolymers obtained were not cytotoxic. In base of the results, the chemical structure of these new copolymers confers a functionality that allows them to serve as nuclei to graft other molecules, such as polysaccharides. Then, the results obtained on the LCST temperature, porosity, interconnected pore network morphology, the ability to be injectable and the biocompatible nature of these copolymers are indicative that these new synthetic biomaterials have the potential to be used in biomedical, pharmacological and for tissue engineering. Also, once their biocompatibility was demonstrate, they may serve to generate interesting compounds having chemical anchor points for the possible addition of polysaccharides using insertion reactions, thereby generating graft copolymers with potential use in biomedical applications

Digital imaging techniques for recording and analysing prehistoric rock art panels in Galicia (NW Iberia)

Several works have highlighted the relevance of 3D modelling techniques for the study of rock art, especially in case of deteriorated state of preservation. This paper presents a methodological approach to accurate document two Bronze Age rock art panels in Galicia (Spain), using photogrammetry SfM. The main aim is to show the application of digital enhancement techniques which have allowed the accurate depiction of the motifs and the correction of previous calques, focusing on the application of the exaggerated shading as a novel analytical method

Resident macrophages of pancreatic islets have a seminal role in the initiation of autoimmune diabetes of NOD mice

Significance Our studies indicate that the resident macrophages of the pancreatic islets of Langerhans have a seminal role in the initiation and progression of autoimmune diabetes in NOD mice. In this study, islet macrophages were depleted by administration of a monoclonal antibody to the CSF-1 receptor. Macrophage depletion, either at the start of the autoimmune process or when diabetogenesis is active, leads to a significant reduction in diabetes incidence. Depletion of the islet macrophages reduces the entrance of T cells into islets and results in the absence of antigen presentation. Concordantly, a regulatory pathway develops that controls diabetes progression. We conclude that treatments that target the islet macrophages may have important clinical relevance for the control of autoimmune type 1 diabetes.</jats:p

Lymphocytes are detrimental during the early innate immune response against Listeria monocytogenes

Mice deficient in lymphocytes are more resistant than normal mice to Listeria monocytogenes infection during the early innate immune response. This paradox remains unresolved: lymphocytes are required for sterilizing immunity, but their presence during the early stage of the infection is not an asset and may even be detrimental. We found that lymphocyte-deficient mice, which showed limited apoptosis in infected organs, were resistant during the first four days of infection but became susceptible when engrafted with lymphocytes. Engraftment with lymphocytes from type I interferon receptor–deficient (IFN-αβR−/−) mice, which had reduced apoptosis, did not confer increased susceptibility to infection, even when the phagocytes were IFN-αβR+/+. The attenuation of innate immunity was due, in part, to the production of the antiinflammatory cytokine interleukin 10 by phagocytic cells after the apoptotic phase of the infection. Thus, immunodeficient mice were more resistant relative to normal mice because the latter went through a stage of lymphocyte apoptosis that was detrimental to the innate immune response. This is an example of a bacterial pathogen creating a cascade of events that leads to a permissive infective niche early during infection

Integrated Planning of Industrial Gas Supply Chains

In this work, we propose a Mixed Integer Linear Programming (MILP) model for optimal planning of industrial gas supply chain, which integrates supply contracts, production scheduling, truck and rail-car scheduling, as well as inventory management under the Vendor Managed Inventory (VMI) paradigm. The objective used here is minimisation of the total operating cost consisting of purchasing of raw material, production, and transportation costs by trucks/rail-cars so as to satisfy customer demands over a given time horizon. The key decisions for production sites include production schedule and purchase schedule of raw material, while the distribution decisions involve customer to plant/depot allocation, quantity transported through rail network, truck delivery amounts, and times. In addition, a relaxation approach is proposed to solve the problem efficiently. An industrial case study is evaluated to illustrate the applicability of the integrated optimisation framework

Recombinant Listeria monocytogenes expressing a cell wall-associated listeriolysin O is weakly virulent but immunogenic

Listeriolysin O (LLO) is an essential virulence factor for the gram-positive bacterium Listeria monocytogenes. Our goal was to determine if altering the topology of LLO would alter the virulence and toxicity of L. monocytogenes in vivo. A recombinant strain was generated that expressed a surface-associated LLO (sLLO) variant secreted at 40-fold-lower levels than the wild type. In culture, the sLLO strain grew in macrophages, translocated to the cytosol, and induced cell death. However, the sLLO strain showed decreased infectivity, reduced lymphocyte apoptosis, and decreased virulence despite a normal in vitro phenotype. Thus, the topology of LLO in L. monocytogenes was a factor in the pathogenesis of the infection and points to a role of LLO secretion during in vivo infection. The sLLO strain was cleared by severe combined immunodeficient (SCID) mice. Despite the attenuation of virulence, the sLLO strain was immunogenic and capable of eliciting protec-tive T-cell responses. Listeria monocytogenes is a gram-positive facultative intra-cellular pathogen extensively used to understand host-patho-gen interactions (44, 51, 53). It expresses the highly conserved pore-forming toxin listeriolysin O (LLO), a member of a large family of cholesterol-dependent cytolysins found in many im

Hierarchical Approach to Integrated Planning of Industrial Gas Supply Chains

In this article, an optimization-based framework is proposed for integrated production and distribution planning of industrial gas supply chains. The main goal is to minimize the overall cost, which is composed of raw material, product sourced from external suppliers, production, truck, and rail-car costs, while satisfying customer demands. The overall problem is formulated as a mixed-integer linear programming (MILP) model while a two-phase hierarchical solution strategy is developed to solve the resulting optimization problem efficiently. The first phase relies on truck scheduling decisions being relaxed, whereas the second phase solves the original model at reduced space by fixing product allocation as determined by phase one. Finally, an industrial-size case study is used to illustrate the applicability and efficiency of the proposed optimization framework