2 research outputs found
Experimental thioacetamide-induced cirrhosis of the liver
Hepatic cirrhosis is a complex disease in
which several biological, biochemical and chemical
alterations are combined, none of these alone being
sufficient for diagnosis. The morphological characteristics
of the final stages of cirrhosis are well known,
but the initial lesions and intermediate stages still have
not been fully clarified.
An experimental model of hepatic cirrhosis by
chronic administration over 30 weeks of thioacetamide
(50 mglkg twice weekly) to female Wistar rats has been
produced. In a macroscopic, microscopic and ultrastructural
study. The different lesions that appeared were
evaluated according to the dose of the toxic agent administered
up, until hepatic cirrhosis was finally installed;
this was after 60 doses of the toxic agent (30 weeks).
Discussion is made of the different types of administration
and the doses employed to obtain a suitable
survival rate for these cases; in our experiments this
was 95%.
It has been demonstrated in both human and experimental
pathology that once the disease itself has been
installed, currently there is no rational or useful
treatment for it. A beneficial effect has been demonstrated
for certain substances, improving the initial and
intermediate lesions. so we conclude by stating that it is
necessary to further study the hepatic lesions preceeding
cirrhosis. Knowledge of these lesions could form the
basis for establishing a useful and rational therapy for
such cases
Down regulation of gastric and intestinal phenotypic expression in Epstein-Barr virus-associated stomach cancers
importance of gastric and intestinal phenotypic
expression for stomach carcinogenesis. In this study, we
focused on Epstein-Barr virus (EBV)-associated
stomach cancers, with special attention to Cdx2.
Methods and Results: We evaluated the expression of
gastric and intestinal phenotypic markers by
immunohistochemistry in 35 EBV-positive [EBV (+)]
and 75 EBV-negative [EBV (-)] stomach cancers in
Colombia. The lesions were divided phenotypically into
gastric (G), gastric-and-intestinal mixed (GI), intestinal
(I), and null (N) phenotypes. In the EBV (+) cases, the
lesions were divided phenotypically into 9 G (25.7%), 1
GI (2.9%), 3 I (8.6%), and 22 N (62.9%) types.
Similarly, the EBV (-) lesions were also classified
phenotypically as 15 G (20.0%), 19 GI (25.3%), 24 I
(32.0%), and 17 N (22.7%) types. The proportion of N
type EBV (+) lesions was higher than for their EBV (-)
counterparts (P<0.0001). The expression of Cdx2 and
MUC2 was also found to be significantly lower in EBV
(+) than in EBV (-) stomach cancers (P=0.0001;
P<0.0001). Cdx2 expression in the intestinal metaplastic
glands present in non-neoplastic mucosa surrounding
EBV (+) lesions was also significantly lower than in
EBV (-) tumors (P=0.016) despite no evidence of EBV
infection by low expression of intestinal phenotype markers,
including Cdx2, and only occasional gastric phenotypic
expression