Evaluation of four different strategies to characterize plasma membrane proteins from banana roots

Plasma membrane proteins constitute a very important class of proteins. They are involved in the transmission of external signals to the interior of the cell and selective transport of water, nutrients and ions across the plasma membrane. However, the study of plasma membrane proteins is challenging because of their poor solubility in aqueous media and low relative abundance. In this work, we evaluated four different strategies for the characterization of plasma membrane proteins from banana roots: (i) the aqueous-polymer two-phase system technique (ATPS) coupled to gelelectrophoresis (gel-based), and (ii) ATPS coupled to LC-MS/MS (gel free), (iii) a microsomal fraction and (iv) a full proteome, both coupled to LC-MS/ MS. Our results show that the gel-based strategy is useful for protein visualization but has major limitations in terms of time reproducibility and efficiency. From the gel-free strategies, the microsomal-based strategy allowed the highest number of plasma membrane proteins to be identified, followed by the full proteome strategy and by the ATPS based strategy. The high yield of plasma membrane proteins provided by the microsomal fraction can be explained by the enrichment of membrane proteins in this fraction and the high throughput of the gel-free approach combined with the usage of a fast high-resolution mass spectrometer for the identification of proteins

Proximity effect on hydrodynamic interaction between a sphere and a plane measured by Force Feedback Microscopy at different frequencies

In this article, we measure the viscous damping $G'',$ and the associated stiffness $G',$ of a liquid flow in sphere-plane geometry in a large frequency range. In this regime, the lubrication approximation is expected to dominate. We first measure the static force applied to the tip. This is made possible thanks to a force feedback method. Adding a sub-nanometer oscillation of the tip, we obtain the dynamic part of the interaction with solely the knowledge of the lever properties in the experimental context using a linear transformation of the amplitude and phase change. Using a Force Feedback Microscope (FFM)we are then able to measure simultaneously the static force, the stiffness and the dissipative part of the interaction in a broad frequency range using a single AFM probe. Similar measurements have been performed by the Surface Force Apparatus with a probe radius hundred times bigger. In this context the FFM can be called nano-SFA

Out of equilibrium anomalous elastic response of a water nano-meniscus

We report the observation of a transition in the dynamical properties of water nano-menicus which dramatically change when probed at different time scales. Using a AFM mode that we name Force Feedback Microscopy, we observe this change in the simultaneous measurements, at different frequencies, of the stiffness G'(N/m), the dissipative coefficient G''(kg/sec) together with the static force. At low frequency we observe a negative stiffness as expected for capillary forces. As the measuring time approaches the microsecond, the dynamic response exhibits a transition toward a very large positive stiffness. When evaporation and condensation gradually lose efficiency, the contact line progressively becomes immobile. This transition is essentially controlled by variations of Laplace pressure

Dephasing by a nonstationary classical intermittent noise

We consider a new phenomenological model for a $1/f^{\mu}$ classical intermittent noise and study its effects on the dephasing of a two-level system. Within this model, the evolution of the relative phase between the $|\pm>$ states is described as a continuous time random walk (CTRW). Using renewal theory, we find exact expressions for the dephasing factor and identify the physically relevant various regimes in terms of the coupling to the noise. In particular, we point out the consequences of the non-stationarity and pronounced non-Gaussian features of this noise, including some new anomalous and aging dephasing scenarii.Comment: Submitted to Phys. Rev.

Integrability of Dirac reduced bi-Hamiltonian equations

First, we give a brief review of the theory of the Lenard-Magri scheme for a non-local bi-Poisson structure and of the theory of Dirac reduction. These theories are used in the remainder of the paper to prove integrability of three hierarchies of bi-Hamiltonian PDE's, obtained by Dirac reduction from some generalized Drinfeld-Sokolov hierarchies.Comment: 15 pages. Corrected some typos and added missing equations in Section 5 for g=sl_n, n>

Glass phases of flux lattices in layered superconductors

We study a flux lattice which is parallel to superconducting layers, allowing for dislocations and for disorder of both short wavelength and long wavelength. We find that the long wavelength disorder has a significant effect on the phase diagram -- it produces a first order transition within the Bragg glass phase and leads to melting at strong disorder. This then allows a Friedel scenario of 2D superconductivity.Comment: 5 pages, 1 eps figure, Revte